Ca2+-induced Ca2+ desensitization of myosin light chain phosphorylation and contraction in phasic smooth muscle
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- 作者：Murahashi ; Tetsuya ; Fujita ; Akikazu ; Kitazawa ; Toshio
- 关键词：Ca2+ ion ; Ca2+ desensitization ; phasic smooth muscle ; phosphorylation ; myosin light chain kinase ; calcium/calmodulin ; dependent protein kinase
- 刊名：Molecular and Cellular Biochemistry
- 出版时间：1999
- 出版年：1999
- 期刊代码：38_03008177
- 类别：cp
- 卷：190
- 期：1/2
- 页码：91-98
- 数据来源：sp
摘要
The temporal relationship between Ca2+-induced contraction and phosphorylation of 20 kDa myosin light chain (MLC) during a step increase in Ca2+ was investigated using permeabilized phasic smooth muscle from rabbit portal vein and guinea-pig ileum at 25°C. We describe here a Ca2+-induced Ca2+ desensitization phenomenon in which a transient rise in MLC phosphorylation is followed by a transient rise in contractile force. During and after the peak contraction, the force to phosphorylation ratio remained constant. Further treatment with cytochalasin D, an actin fragmenting agent, did not affect the transient increase in phosphorylation, but blocked force development. Together, these results indicate that the transient phosphorylation causes the transient contraction and that neither inhomogeneous contractility nor reduced thin filament integrity effects the transient phosphorylation. Lastly, we show that known inhibitors to MLC kinase kinases and to a Ca2+-dependent protein phosphatase did not eliminate the desensitized contractile force. This study suggests that the Ca2+-induced Ca2+ desensitization phenomenon in phasic smooth muscle does not result from any of the known intrinsic mechanisms involved with other aspects of smooth muscle contractility.