Atomic force microscopy of microvillous cell surface dynamics at fixed and living alveolar type II cells
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- 作者：Elena Hecht (1)
Shariq M. Usmani (2)
Susanne Albrecht (2)
Oliver H. Wittekindt (2) oliver.wittekindt@uni-ulm.de
Paul Dietl (2)
Boris Mizaikoff (1)
Christine Kranz (1) christine.kranz@uni-ulm.de - 关键词：Atomic force microscopy – ; ATII cells – ; Microvilli – ; Scanning electron microscopy – ; Confocal microscopy – ; Capsazepine (CPZ) – ; 2 ; Aminoethoxydiphenyl ; borate (2 ; APB) – ; Lung – ; Epithelium
- 刊名：Analytical and Bioanalytical Chemistry
- 出版时间：March 2011
- 出版年：2011
- 期刊代码：8_1618-2642
- 类别：en
- 卷：399
- 期：7
- 页码：2369-2378
- 数据来源：sp
摘要
Scanning probe techniques enable direct imaging of morphology changes associated with cellular processes at life specimen. Here, glutaraldehyde-fixed and living alveolar type II (ATII) cells were investigated by atomic force microscopy (AFM), and the obtained topographical data were correlated with results obtained by scanning electron microscopy (SEM) and confocal microscopy (CM). We show that low-force contact mode AFM at glutaraldehyde-fixed cells provides complementary results to SEM and CM. Both AFM and SEM images reveal fine structures at the surface of fixed cells, which indicate microvilli protrusions. If ATII cells were treated with Ca2+ channel modulators known to induce massive endocytosis, changes of the cell surface topography became evident by the depletion of microvilli. Low force contact mode AFM imaging at fixed ATII cells revealed a significant reduction of the surface roughness for capsazepine and 2-aminoethoxydiphenyl-borate (CPZ/2-APB)-treated cells compared to untreated control cells (Rc of 99.7 卤 6.8 nm vs. Rc of 71.9 卤 4.6 nm for N = 22), which was confirmed via SEM studies. CM of microvilli marker protein Ezrin revealed a cytoplasmic localization of Ezrin in CPZ/2-APB-treated cells, whereas a submembranous Ezrin localization was observed in control cells. Furthermore, in situ AFM investigations at living ATII cells using low force contact mode imaging revealed an apparent decrease in cell height of 17%during stimulation experiments. We conclude that a dynamic reorganization of the microvillous cell surface occurs in ATII cells at conditions of stimulated endocytosis.