L-Rhamnose induction of Aspergillus nidulans 伪-L-rhamnosidase genes is glucose repressed via a CreA-independent mechanism acting at the level of inducer uptake
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- 作者：Juan A Tamayo-Ramos (12) juan.tamayoramos@wur.nl
Michel Flipphi (1) drir.michelflipphi@gmail.com
Ester Pardo (1) epardo@iata.csic.es
Paloma Manzanares (1) pmanz@iata.csic.es
Margarita Orejas (1) morejas@iata.csic.es - 关键词：Aspergillus nidulans – ; Carbon catabolite repression – ; CreA ; independent – ; Inducer exclusion – ; α ; L ; rhamnosidase – ; Transcriptional regulation
- 刊名：Microbial Cell Factories
- 出版时间：December 2012
- 出版年：2012
- 期刊代码：pc366_1475-2859
- 类别：ch
- 卷：11
- 期：1
- 页码：DOI: 10.1186/1475-28
- 数据来源：sp
摘要
Background Little is known about the structure and regulation of fungal α-L-rhamnosidase genes despite increasing interest in the biotechnological potential of the enzymes that they encode. Whilst the paradigmatic filamentous fungus Aspergillus nidulans growing on L-rhamnose produces an α-L-rhamnosidase suitable for oenological applications, at least eight genes encoding putative α-L-rhamnosidases have been found in its genome. In the current work we have identified the gene (rhaE) encoding the former activity, and characterization of its expression has revealed a novel regulatory mechanism. A shared pattern of expression has also been observed for a second α-L-rhamnosidase gene, (AN10277/rhaA).