Evaluation of Genetic Variation in the Clown Knifefish, Chitala chitala , Using Allozymes, RAPD, and Microsatellites
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- 作者：Anup Mandal ; Kuldeep Kumar Lal ; Vindhya Mohindra ; Rajeev Kumar Singh ; Peyush Punia ; U. K. Chauhan and Wazir Singh Lakra
- 关键词：Chitala chitala ; Genetic diversity ; Allozymes ; Microsatellites ; RAPD
- 刊名：Biochemical Genetics
- 出版时间：April, 2009
- 出版年：2009
- 期刊代码：11_00062928
- 类别：mat
- 卷：47
- 期：3-4
- 页码：216-234
- 数据来源：sp
摘要
Twenty-seven enzyme systems, six random amplified polymorphic DNA (RAPD) primers, and two microsatellite loci were tested to determine intraspecific divergence in the natural population of the endangered Indian featherback fish, Chitala chitala, for the first time. The 262 samples of C. chitala were collected from six riverine locations in India: the Satluj, Ganga (Ghagra, Bhagirathi, and Brahmaputra), Mahanadi, and Narmada river systems. The analysis revealed population subdivisions, with an FST value from 0.1235 (95%confidence 0.0868–0.1621) for RAPD and a combined FST of 0.0344 (95%confidence 0.0340–0.0350) for microsatellite loci. An analysis of 38 allozyme loci did not reveal any polymorphism in the samples from any of the riverine localities; a possible explanation for this could be that the ancestors of Chitala could have faced a population reduction in prehistoric periods, as low allozyme variation is also reported for other species of Chitala from south Asia.