The Ty1-copia group of retrotransposons in plants: genomic organisation, evolution, and useas molecular markers
| 详细信息 | 推荐本文 | |
- 作者：Kumar ; Amar ; Pearce ; Stephen R. ; McLean ; Karen ; Harrison ; Gill ; Heslop Harrison ; J.S. ; Waugh ; Robbie ; Flavell ; Andrew J.
- 关键词：Ty1 ; copia ; retrotransposons ; retroelements ; plants ; genomic organisation ; evolution ; molecular marker
- 刊名：Genetica
- 出版时间：1997
- 出版年：1997
- 期刊代码：28_00166707
- 类别：ear
- 卷：100
- 期：1/3
- 页码：205-217
- 数据来源：sp
摘要
The genomic organisation and diversity of the Ty1-copia group retrotransposons has been investigatedin several crop plants and their relatives from bothdicotyledonous and monocotyledonous families, includingpotato ( Solanum tuberosum), faba beans ( Viciafaba), Vicia melanops, Vicia sativa, barley (Hordeum vulgare), rye ( Secale cereale), and onion( Allium cepa). Extreme heterogeneity in thesequence of the Ty1-copia retrotransposons from allthese plants was revealed following sequence analysis ofreverse transcriptase fragments. The estimated copynumbers of the Ty1-copia group retrotransposons forthe genomes of S. tuberosum, L. esculentum, A. cepa,S. cereale, and V. faba is highly variable, rangingfrom a few hundred to approximately a million copies pergenome. In situ hybridisation data from metaphaseand prophase chromosomes of V. faba, S. cereale,and H. vulgare suggest that retrotransposonsequences are dispersed throughout the euchromaticregions of the genome but are almost undetectable inmost heterochromatic regions. In contrast, similar datafrom metaphase chromosomes of A. cepa suggeststhat although retrotransposon sequences are dispersedthroughout the euchromatic regions of the genome, theyare predominantly concentrated in the terminalheterochromatin. These results are discussed in thecontext of the role played by the Ty1-copia groupretrotransposons in the evolution of the plant genome.Lastly, the application of retrotransposon sequences asgenetic markers for mapping genomes and for studyinggenetic biodiversity in plants is presented.