The tms2 gene as a negative selection marker in rice

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• 作者：Narayana M. Upadhyaya ; Xue-Rong Zhou ; Limin Wu ; Kerrie Ramm and Elizabeth S. Dennis
• 关键词：IAAH ; negative selector ; rice ; tms2 gene
• 刊名：Plant Molecular Biology Reporter
• 出版时间：September, 2000
• 出版年：2000
• 期刊代码：47_07359640
• 类别：cp
• 卷：18
• 期：3
• 页码：227-233
• 数据来源：sp

摘要

A conditional negative selection marker is essential for high throughput insertional mutagenesis with any two-element transposon tagging system. Thetms2 gene encodes indoleacetic acid hydrolase (IAAH) which converts naphthaleneacetamide (NAM) to the potent auxin naphthaleneacetic acid, a phytotoxic derivative. This gene, under the control of the manopine synthase gene 2 promoter fromAgrobacterium tumefaciens and exogenously applied NAM, have been used effectively as a negative selector inAc/Ds insertional mutagenesis ofArabidopsis thaliana (Sundaresan et al., 1995). In this study we show thattms2 can also be used as a negative selector in rice. T₁ transgenic seedlings expressing thistms2 gene under the control of themas2’ promoter showed significant reduction in shoot and root growth in the presence of 5–10 μM NAM under specified growth conditions compared to plants not containing this gene.