Global Chemome Study by LC Coupled with DAD and ESI鈥換鈥揟OF MS of a Composite Traditional Chinese Medicine Qishenyiqi Dropping Pills
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- 作者：Jing Wang ; Na Wei ; Hongzhi Zhao ; Hao Huang ; Min Jiang ; Gang Bai and Guoan Luo
- 关键词：Column liquid chromatography ; DAD– ; Q– ; TOF mass spectrometry ; Chemomics ; Global chemome ; Sub ; chemome ; Traditional Chinese medicine prescription
- 刊名：Chromatographia
- 出版时间：September 2010
- 出版年：2010
- 期刊代码：19_0009-5893
- 类别：ph
- 卷：72
- 期：5-6
- 页码：431-440
- 数据来源：sp
摘要
High-performance liquid chromatography coupled with diode-array detection and electrospray ionization quadrupole-time of flight mass spectrometry (ESI–Q–TOF MS) has been used for global chemome analysis of Qishenyiqi dropping pills. Under gradient conditions, two sub-chemomes extracted from Qishenyiqi dropping pills were analyzed on the same C18 reversed-phase column by use of different binary mobile phases. In the chromatogram obtained from sub-chemome I, 19 compounds with strong ultraviolet absorption at 260 nm were detected; 27 compounds with weak ultraviolet absorption at 203 nm were detected in the chromatogram obtained from sub-chemome II. All the compounds were identified by ESI–Q–TOF MS. This study furnished much more important information about the pharmacological activity of Qishenyiqi dropping pills than previous studies and laid a good foundation for further exploration of chemomics.