Voltammetric analysis of metallothioneins and copper (II) in fish for water biomonitoring studies
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- 作者：1. REQUIMTE ; Department of Chemistry and Biochemistry ; Faculty of Sciences ; University of Porto ; Rua do Campo Alegre ; 687 ; 4169-007 Porto ; Portugal
- 关键词：Metallothioneins – ; Copper (Cu) – ; Biomarkers – ; Square ; wave voltammetry (SWV) – ; Heavy metal biomonitoring
- 刊名：Environmental Chemistry Letters
- 出版时间：September 2011
- 出版年：2011
- 期刊代码：36_1610-3653
- 类别：ch
- 卷：9
- 期：3
- 页码：405-410
- 数据来源：sp
摘要
An major research area in environmental chemistry is the development of methods for the analysis of biomarkers. Metallothioneins are used as biomarkers in studies of heavy metals exposure in water, because metallothioneins are synthesized and accumulated when organisms are exposed to toxic concentrations of pollutants. In this work, simple and sensitive voltammetric methods were developed for metallothionein and copper (II) determinations in fish liver Lepomis gibbosus. Both analytical methodologies were optimized and applied to samples extracted from individuals previously submitted to sub-lethal toxicological trials with copper sulphate (CuSO4) and cadmium chloride (CdCl2). The obtained results showed that both methods are very precise, sensitive, and involve simple sample preparation processes. Moreover, metallothioneins showed better correlation with the toxic exposure than Cu2+. To the best of our knowledge, this is the first time that hepatic metallothioneins and Cu2+ contents are voltammetrically determined in order to be compared in their function as heavy metal biological indicators.