Transformed Roots of Lupinus mutabilis: Induction, Culture and Isoflavone Biosynthesis

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• 作者：Babaoglu ; Mehmet ; Davey ; Michael R. ; Power ; J. Brian ; Sporer ; Frank ; Wink ; Michael
• 关键词：Agrobacterium rhizogenes ; A. tumefaciens ; genistein ; genistin ; grain legumes ; 2&#8242 ; hydroxygenistein ; isoflavones ; NPTII ELISA ; secondary metabolites ; Southern hybridisation ; wighteone
• 刊名：Plant Cell ; Tissue and Organ Culture
• 出版时间：2004
• 出版年：2004
• 期刊代码：58_01676857
• 类别：ch
• 卷：78
• 期：1
• 页码：29-36
• 数据来源：sp

摘要

Transformed roots of Lupinus mutabilis cv. Potosi induced by Agrobacterium rhizogenes strain R1601 were cultured on Murashige and Skoog-based medium lacking kanamycin sulphate, or with this antibiotic at 40 mg l⁻¹. The neomycin phosphotransferase gene in the genome of transformed roots was confirmed by non-radioactive Southern hybridisation. Neomycin phosphotransferase protein was detected by ELISA. Transformed roots synthesised isoflavones, but not quinolizidine alkaloids; the latter are typical secondary metabolites of lupin normally produced in aerial parts of the plant. Genistein and 2’-hydroxygenistein, were the main secondary metabolites in cultured, transformed roots, whereas the glycoside genistin was more abundant in roots of non-transformed plants. Wighteone concentrations in transgenic roots were higher than those of non-transformed roots. Transformed roots produced twice the concentration of isoflavones compared with roots from non-transformed plants, indicating that Ri plasmid T-DNA genes modified isoflavone concentration and pattern of biosynthesis.