Glutamate Differently Modulates Metabotropic Glutamate Receptors in Neuronal and Glial Cells
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- 作者：Carlos Alberto Castillo ; David Agust铆n Le贸n ; Inmaculada Ballesteros-Y谩帽ez ; Inmaculada Iglesias ; Mairena Mart铆n and Jos茅 Luis Albasanz
- 关键词：Excitotoxicity ; Metabotropic glutamate receptors ; Neurons ; C6 glioma cells ; l ; Glutamate
- 刊名：Neurochemical Research
- 出版时间：July, 2010
- 出版年：2010
- 期刊代码：43_03643190
- 类别：cp
- 卷：35
- 期：7
- 页码：1050-1063
- 数据来源：sp
摘要
Glutamate is an excitatory neurotransmitter implicated in learning and memory processes, but at high concentrations it acts as an excitotoxin causing degeneration and neuronal death. The aim of this work was to determine the excitotoxic effect of glutamate and the regulation of metabotropic glutamate receptors (mGluR) during excitotoxicity in neurons and C6 glioma cells. Results show that glutamate causes excitotoxic damage only in cortical neurons. Loss of cell viability in neurons was glutamate concentration- and time-dependent. Total mGluR levels were significantly reduced in these cells when exposed to glutamate. However, in C6 cells, which have been used as a model of glial cells, these receptors were regulated in a biphasic manner, decreased after 6 h, and increased after 24/48 h of treatment. Results show a cell dependent mGluR regulation by glutamate exposure which could mediate the vulnerability or not to glutamate mediated excitotoxicity.