Determination of glycated hemoglobin on the basis of spectral shifting from protein-dye interaction
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- 作者：Eun Joong Kim (1)
Seung Yeon Song (1)
Bun Yeoul Lee (1)
Hyun C. Yoon (1) hcyoon@ajou.ac.kr - 关键词：Glycated hemoglobin ; Glycated protein ; Hemoglobin A1c ; Optical sensing ; Spectral shifting
- 刊名：BioChip Journal
- 出版时间：March 2010
- 出版年：2010
- 期刊代码：3_2092-7843
- 类别：ch
- 卷：4
- 期：1
- 页码：16-21
- 数据来源：sp
摘要
A method for the detection of glycated proteins was developed on the basis of spectral shifting observed for 1-(4-Boronophenylazo)-2-hydroxy-3,6-naphthalene-disulfonic acid (azo-BA) dye. Hemoglobin A1c, a type of glycated hemoglobin, is an important indicator of diabetes mellitus control and management. We have employed the azo-BA dye containing a boronic acid group linked to an aromatic chromophore. The boronic acid group of the dye specifically binds to the carbohydrate chain of HbA1c via cis-diol interaction, and its spectra exhibit a unique chromatic redshift. Because the reaction is spontaneous at neutral pH, this method provides an advantage of short detection time. After the azo-BA dye/HbA1c reaction commenced, the concentration of conjugate was measured by registering spectral shifts. The measurements revealed that the method has a dynamic detection range of 3–15%(HbA1c/total hemoglobin), which covers the required clinical reference range. This method has shown reproducible results; the coefficient of variation of the test was 3.60%. Thus, the proposed method was found suitable for measuring%HbA1c and would be applicable for diabetes mellitus control.