A new method for evaluating s2 ligand activity in the isolated guinea-pig bladder
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- 作者：Nicola A. Colabufo ; Francesco Berardi ; Marialessandra Contino ; Roberto Perrone and Vincenzo Tortorella
- 关键词：Guinea ; pig bladder ; Guinea ; pig ileum ; 2 receptor activity ; 1 receptor desensitization
- 刊名：Naunyn-Schmiedeberg's Archives of Pharmacology
- 出版时间：August 2003
- 出版年：2003
- 期刊代码：190_0028-1298
- 类别：bio
- 卷：368
- 期：2
- 页码：106-112
- 数据来源：sp
摘要
We demonstrated the presence of 2receptors in the guinea-pig ileum by saturation analysis and extended our investigation to guinea-pig bladder and rat bladder. In functional assays of the isolated tissues in organ baths, 2 receptor ligands inhibited electrically evoked contractions in both guinea-pig bladder and ileum and a linear correlation was found between 2 receptor affinity and 2 receptor activity values of selected compounds. The 2 activity of these compounds in the presence of desensitised 1 receptors both in bladder and ileum was also tested. On the basis of our results, we propose the electrically stimulated guinea-pig bladder as a new method for evaluating 2 activity.