Preferential utilization of petroleum oil hydrocarbon components by microbial consortia reflects degradation pattern in aliphatic鈥揳romatic hydrocarbon binary mixtures
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- 作者：Hernando Pactao Bacosa (1) bacosa@er.kankyo.tohoku.ac.jp
Koichi Suto (1)
Chihiro Inoue (1) - 关键词：Biodegradation – ; Microbial consortium – ; Aliphatic hydrocarbons – ; Aromatic hydrocarbons – ; Binary mixture – ; Petroleum
- 刊名：World Journal of Microbiology and Biotechnology
- 出版时间：May 2011
- 出版年：2011
- 期刊代码：121_09593993
- 类别：bio
- 卷：27
- 期：5
- 页码：1109-1117
- 数据来源：sp
摘要
In this study, the abilities of two microbial consortia (Y and F) to degrade aliphatic–aromatic hydrocarbon mixtures were investigated. Y consortium preferentially degraded the aromatic hydrocarbon fractions in kerosene, while F consortium preferentially degraded the aliphatic hydrocarbon fractions. Degradation experiments were performed under aerobic conditions in sealed bottles containing liquid medium and n-octane or n-decane as representative aliphatic hydrocarbons or toluene, ethylbenzene or p-xylene as representative aromatic hydrocarbons (all at 100 mg/l). Results demonstrated that the Y consortium degraded p-xylene more rapidly than n-octane. It degraded toluene, ethylbenzene and p-xylene more rapidly than decane. In comparison, the F consortium degraded n-octane more rapidly than toluene, ethylbenzene or p-xylene, and n-decane more rapidly than toluene, ethylbenzene or p-xylene. 16S rRNA gene sequencing revealed that the Y consortium was dominated by Betaproteobacteria and the F consortium by Gammaproteobacteria, and in particular Pseudomonas. This could account for their metabolic differences. The substrate preferences of the two consortia showed that the aliphatic–aromatic hydrocarbon binary mixtures, especially the n-decane–toluene/ethylbenzene/p-xylene pairs, reflected their degradation ability of complex hydrocarbon compounds such as kerosene. This suggests that aliphatic–aromatic binary systems could be used as a tool to rapidly determine the degradation preferences of a microbial consortium.