Long-term influence of the presence of a non-aqueous phase on the cell surface hydrophobicity of Pseudomonas in two-phase partitioning bioreactors

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• 作者：Mar铆a Hern谩ndez (1)
  Ra煤l Mu帽oz Torre (1) mutora@iq.uva.es
• 关键词：Cell surface hydrophobicity &#8211 ; 2 ; 2 ; 4 ; 4 ; 6 ; 8 ; 8 ; heptamethylnonane &#8211 ; Pseudomonas &#8211 ; Silicone oil &#8211 ; Two ; phase partitioning bioreactors
• 刊名：Applied Microbiology and Biotechnology
• 出版时间：March 2011
• 出版年：2011
• 期刊代码：18_0175-7598
• 类别：bio
• 卷：89
• 期：5
• 页码：1573-1581
• 数据来源：sp

摘要

The long-term influence of silicone oil 200 cSt (SO200) and 2, 2, 4, 4, 6, 8, 8-heptamethylnonane (HMN) on the cell surface hydrophobicity (CSH) of a hexane-degrading Pseudomonas aeruginosa strain and a toluene-degrading Pseudomonas putida strain was assessed in two-phase partitioning bioreactors under batch and continuous operation. CSH was evaluated using a modified BATH method based on optical density (CSH_OD) and colony-forming unit (CSH_CFU) measurements. In the presence of HMN, P. aeruginosa turned hydrophobic over the time course as shown by the gradual increase in CSH_OD (61 卤 1%) and CSH_CFU (53 卤 3%) under batch degradation and in CSH_OD (49 卤 0%) under continuous operation. However, P. putida turned hydrophobic only under continuous operation (). On the other hand, no significant CSH enhancement was observed in both Pseudomonas strains in the presence of SO200. These results suggested that CSH is species, non-aqueous phase, and cultivation mode dependant, and an inducible property of bacteria. Maximum hexane elimination capacities increased by 2 and 3 in the presence of SO200 and HMN, respectively. Based on the absence of CSH in P. aeruginosa in the presence of SO200, the higher elimination capacities recorded were likely due to an improved hexane mass transfer (physical effect). However, in the presence of HMN, a direct hexane uptake from the non-aqueous phase (biological effect) might have also contributed to this enhancement.