Interaction of Salivary and Midgut Proteins of Helicoverpa armigera with Soybean Trypsin Inhibitor
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- 作者：Santosh Kumar Upadhyay (1)
Krishnappa Chandrashekar (1) kc_shekar2001@yahoo.com - 关键词：Soybean trypsin inhibitor – ; Helicoverpa armigera – ; Protease – ; Affinity column – ; Zymogram
- 刊名：The Protein Journal
- 出版时间：March 2012
- 出版年：2012
- 期刊代码：221_1572-3887
- 类别：bio
- 卷：31
- 期：3
- 页码：259-264
- 数据来源：sp
摘要
Feeding of Helicoverpa armigera larvae on semi-synthetic diet containing Soybean trypsin inhibitor (STI) resulted in disappearance of STI sensitive protease in salivary and midgut protease extract. This might be due to in situ inhibition by dietary STI. STI was largely degraded within 1 h of incubation with total salivary protease (1:1). Degradation was relatively low in midgut proteases. STI interacting proteins were isolated from saliva and midgut extracts of larvae fed on STI supplemented diet using affinity column. Most of the isolated proteins showed caseinolytic activity in zymogram. Denovo sequencing data of seven different peptides selected from trypsin digested total protein showed similarity to chymotrypsinogen, serine protease, aminopeptidase N, peroxidase, hypothetical protein and muscle specific protein.