Up-regulation of tRNA biosynthesis affects translational readthrough in MALL>maf1-? mutant of Saccharomyces cerevisiae
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- 作者：Marta Kwapisz ; Wies艂aw J. Smagowicz ; Danuta Oficjalska ; Isabelle Hatin ; Jean-Pierre Rousset ; Teresa 呕o艂膮dek and Magdalena Boguta
- 刊名：Current Genetics
- 出版时间：December 2002
- 出版年：2002
- 期刊代码：63_0172-8083
- 类别：bio
- 卷：42
- 期：3
- 页码：147-152
- 数据来源：sp
摘要
Maf1p is a negative effector of RNA polymerase III in yeast. The maf1-( mutation caused an increase in the level of cellular tRNAs, but a decrease of translational readthrough at nonsense codons. Using the lacZ-luc dual gene reporter system, we detected an almost twofold diminution of UAA and UAG readthrough in maf1-( compared with the parental strain. The maf1-( mutation did not affect the rate of protein biosynthesis and growth at standard conditions, but resulted in temperature-sensitive growth on non-fermentable carbon sources. We examined the correlation of the temperature sensitive and antisuppression phenotypes of maf1-( using a colour phenotype assay in the ade2-1 SUP11 strain. Antisuppression, but not the temperature-sensitive growth defect, was compensated either by increased dosage of SUP11 or by [PSI+], the prion form of the translation termination factor Sup35p. Summarizing, the elevated tRNA levels in maf1-( increase translational fidelity and, independently, affect growth under special conditions.