Enhanced glutathione S-transferase expression in 2-hydroxyamino- 1-methyl-6-phenylimidazo[4,5-b]pyridine-resistant IEC-18 cells
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- 作者：W. Teubner ; J. I. Fuchs and P. Steinberg
- 关键词：2 ; amino ; 1 ; methyl ; 6 ; phenylimidazo[4 ; 5 ; b]pyridine ; glutathione S ; transferase activity ; glutathione S ; transferase subunit expression ; heterocyclic aromatic amines ; 2 ; hydroxyamino ; 1 ; methyl ; 6 ; phenylimidazo[4 ; 5 ; b]pyridine ; IEC ; 18 cells
- 刊名：Cell Biology and Toxicology
- 出版时间：May, 2007
- 出版年：2007
- 期刊代码：4_07422091
- 类别：cp
- 卷：23
- 期：3
- 页码：153-161
- 数据来源：sp
摘要
In the present study we show that repeated exposure of the rat intestinal epithelial cell line IEC-18 to 2-hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine (N-OH-PhIP), from a toxicological point of view the most relevant phase-1 metabolite of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP, the main heterocyclic aromatic amine present in processed meat), led to the selection of N-OH-PhIP-resistant IEC-18 cells. This phenomenon was accompanied by a fivefold increase in total glutathione S-transferase (GST) activity, measured with the broad-spectrum substrate 1-chloro-2,4-dinitrobenzene, in the N-OH-PhIP-resistant IEC-18 cells. Furthermore, a Western blotting analysis revealed that the expression of GST subunits A1, A3, A4, M1 and P1 was enhanced in the N-OH-PhIP-resistant IEC-18 cells.