Decreased peroxidase activity in transgenic tobacco and its effect on lignification
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- 作者：Tijen Talas-O臒ra艧 ; Kemal Kazan and Nermin G枚z眉k谋rm谋z谋
- 关键词：antisense expression ; lignification ; peroxidase
- 刊名：Biotechnology Letters
- 出版时间：2001
- 出版年：2001
- 期刊代码：6_01415492
- 类别：en
- 卷：23
- 期：4
- 页码：267-273
- 数据来源：sp
摘要
An antisense gene construct of a peroxidase gene (Shpx6a) from a tropical pasture legume Stylosanthes humilis was transferred into tobacco cells via Agrobacterium tumefaciens to test whether peroxidase activity could be decreased and what effect this would have on lignification. A large number of tobacco cell lines were regenerated on selective media and stable integration of the transgene was confirmed in randomly selected putative transformants. Analyses of the primary transgenic plants and their progeny (T1) demonstrated that the total peroxidase activity was significantly decreased (up to 36%) as compared to that measured in untransformed control plants. Importantly, reduction in peroxidase activity is accompanied by decreases (up to 23%) in lignin content in several transgenic lines.