Estrogen directly down-regulates the bone-resorbing activity of mature osteoclasts through nuclear estrogen receptor α
| 详细信息 | 推荐本文 | |
- 作者：Hiroshi Mano ; Yoshiyuki Hakeda and Masayoshi Kumegawa
- 关键词：estrogen ; estrogen receptor ; gene expression ; osteoclast ; osteoporosis ; pit assay
- 刊名：Cytotechnology
- 出版时间：2001
- 出版年：2001
- 期刊代码：8_09209069
- 类别：soc
- 卷：35
- 期：1
- 页码：17-23
- 数据来源：sp
摘要
The decrease in estrogen level that follows the onset ofmenopause causes rapid bone loss, resulting in osteoporosis.However, the mechanism remains unclear, especially concerningthe regulation of bone-resorbing osteoclasts. Here we analyzedthe function of estrogen and its receptor in matureosteoclasts. We found that estrogen directly inhibitedbone-resorption by purified rabbit mature-osteoclasts.Moreover, using a RT-PCR technique, we report that nuclearestrogen receptor (ER) but not ER is expressed in mature osteoclasts. The antisense oligodeoxynucleotide for ER inhibited the reductionin osteoclastic bone-resorbing activity caused by estrogen. We conclude that in part estrogen directly inhibits the bone-resorbing activity of mature osteoclasts through the ER.