ACL reconstruction in a rabbit model using irradiated Achilles allograft seeded with mesenchymal stem cells or PDGF-B gene-transfected mesenchymal stem cells
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- 作者：Feng Li ; Hongti Jia and Changlong Yu
- 关键词：Mesenchymal stem cells ; Allograft ; Anterior cruciate ligament ; PDGF ; BB ; Retrovirus
- 刊名：Knee Surgery ; Sports Traumatology ; Arthroscopy
- 出版时间：October, 2007
- 出版年：2007
- 期刊代码：143_0942-2056
- 类别：med
- 卷：15
- 期：10
- 页码：1219-1227
- 数据来源：sp
摘要
The present study was conducted to develop a new strategy to accelerate reconstruction of the anterior cruciate ligament (ACL) by modifying the Achilles allograft with autogenous mesenchymal stem cells (MSCs) or PDGF-B transfected MSCs in a rabbit model. The allografts were first irradiated with Co60, stored at −80掳C, and then seeded with cells for implantation. Bilateral ACL reconstructions were performed. On the left, the allograft was either seeded with MSCs or PDGF-B transfected MSCs and acted as the experimental group. On the right, the graft without any cells seeded acted as control. At 3, 6 and 12 weeks after surgery, histological observation found that implatation of MSCs or PDGF-B transfected MSCs accelerated cellular infiltration into the ACL and enhanced collagen deposition in the wound. PDGF-B transfected MSCs could also lead to an initial promotion of angiogenesis. This gene transfer technique or cell implantion may be a potentially useful tool for improving ligament remodeling.