Improvement in thermal stability of l-fucose dehydrogenase by chemical modification and additives for use as an NADPH recycling reagent
| 详细信息 | 推荐本文 | |
- 作者：Mikio Bakke ; Keisuke Furukawa and Naoki Kajiyama
- 关键词：chelating reagents ; chemical modification ; fucose dehydrogenase ; NADPH recycling ; stabilization
- 刊名：Biotechnology Letters
- 出版时间：2002
- 出版年：2002
- 期刊代码：6_01415492
- 类别：en
- 卷：24
- 期：13
- 页码：1075-1078
- 数据来源：sp
摘要
Chemical modification and addition of stabilizers were evaluated to stabilize l-fucose dehydrogenase (FDH) in mild alkaline which is required for NADPH recycling used in a glutamate dehydrogenase (GDH)-catalyzed elimination of ammonia. FDH lost all its activity at pH 9 after 10 min at 50 °C, while FDH conjugated with dextran retained about 30%activity. EDTA or citric acid, at 50–100 mM, increased the thermal stability of FDH with retention of more than 70–80%activity after heat-treatment. FDH was then functional for NADPH regeneration.