Different protein expression of myocardium from Chinese mini-swine model of myocardial infarct
| 详细信息 | 推荐本文 | |
- 作者：Yanfeng Zhao (1)
Nengsheng Ye (1)
Rongli Zhang (1)
Xue Feng (1)
Guoan Luo (1) luoga@mail.tsinghua.edu.cn
Yiming Wang (1) - 关键词：myocardial infarct ; myocardial tissue ; proteome ; two ; dimensional gel electrophoresis
- 刊名：Frontiers of Chemistry in China
- 出版时间：October 2007
- 出版年：2007
- 期刊代码：pc337_1673-3495
- 类别：ch
- 卷：2
- 期：4
- 页码：422-427
- 数据来源：sp
摘要
High-resolution two-dimensional gel electrophoresis (2-DE), followed by computer-assisted image analysis was used to screen protein patterns of normal and infarcted myocardial tissues for quantitative and qualitative differences in protein expression. In the gels of pH 5–8 immobilized pH gradient (IPG) strips, 851 protein spots were detected in normal myocardial tissue and 1 032 protein spots were resolved in infarcted myocardial tissue. Thirteen protein spots only expressed in normal myocardial tissue, and 14 protein spots only expressed in infarcted myocardial tissue. Results also showed that 49 protein spots displayed quantitative changes in expression between normal and infarcted myocardial tissue. Eleven protein spots were subjected to mass spectrometry (MS) analysis and seven proteins were identified by peptide mass fingerprinting (PMF). These proteins may be involved in cardiovascular injury, and could play an important role in the treatment of coronary heart disease.