Dysregulation of blood lymphocyte subsets and natural killer cells in schistosomal liver cirrhosis and hepatocellular carcinoma
| 详细信息 | 推荐本文 | |
- 作者：A.M. Attallah A1 ; A.A. Tabll A2 A3 ; M. El-Sadany A2 ; T.A. Ibrahim A2 ; I. El-Dosoky A2
- 关键词：Hepatocellular carcinoma ; Liver cirrhosis ; Lymphocyte subsets ; Natural killer cells ; Flow cytometry
- 刊名：Clinical and Experimental Medicine
- 出版时间：September 2003
- 出版年：2003
- 期刊代码：35_1591-8890
- 类别：med
- 卷：3
- 期：3
- 页码：181-185
- 数据来源：sp
摘要
Immunological factors are important in the pathogenesis of a wide spectrum of hepatobiliary diseases. Using flow cytometry, we determined the changes in lymphocyte subsets and natural killer cells in 123 individuals (81 patients with liver disease and 42 healthy volunteers). The liver diseases included periportal fibrosis (PPF, 10 patients), liver cirrhosis (LC, 31 patients), and hepatocellular carcinoma (HCC, 40 patients). Schistosomiasis and viral hepatitis B and C were the putative etiological agents of liver diseases. Immunophenotyping by indirect immunofluorescence was conducted using monoclonal antibodies to CD3 (T-lymphocytes), CD4 (helper/inducer T-cells), CD8 (suppressor/cytotoxic T-cells), and CD57 (natural killer cells) cell surface markers. Immunophenotyping of PPF patients showed no significant changes in all markers compared with the healthy controls. However, there was a significant decrease ( P<0.01) in CD3 and CD4 T-cells, and a highly significant increase ( P<0.001) in CD57 T-cells in patients with LC or HCC. In addition, LC and HCC patients showed no significant change in CD8 T-cells compared with controls. In conclusion, the progression of liver diseases is associated with a dysregulation of cellular immune responses. T-lymphocytes and natural killer cells may play a role in the immunopathogenesis of liver cirrhosis and HCC.