Cell-culture compatible silk fibroin scaffolds concomitantly patterned by freezing conditions and salt concentration
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- 作者：Fr茅d茅ric Byette (1)
Fr茅d茅ric Bouchard (1)
Christian Pellerin (2)
Joanne Paquin (1)
Isabelle Marcotte (13) marcotte.isabelle@uqam.ca
Mircea A. Mateescu (13) mateescu.m-alexandru@uqam.ca - 关键词：Silk fibroin – ; Scaffold – ; Cell culture – ; Freeze ; drying – ; Crystal nucleation and growth – ; Sodium chloride concentration – ; Freezing temperature – ; Mechanical properties – ; Spectroscopic characterization
- 刊名：Polymer Bulletin
- 出版时间：June 2011
- 出版年：2011
- 期刊代码：pc374_0170-0839
- 类别：ch
- 卷：67
- 期：1
- 页码：159-175
- 数据来源：sp
摘要
The morphology of freeze-dried silk fibroin 3D-scaffolds was modified by varying both the NaCl concentration and the freezing temperature of the silk fibroin solution prior to lyophilization. Scanning electron micrographs showed that slow freezing at −22 掳C generated sponge-like interconnected porous networks, whereas fast freezing at −73 掳C formed stacked leaflet structures. The presence of millimolar NaCl (50–250 mM) increased the porosity of the scaffolds and generated small outgrowths at their surface, depending on the freezing regime. Our results suggest that the morphological differences seen between the materials likely depend on ice and NaCl hydrate crystal nucleation and growth mechanisms. Infrared spectroscopy and X-ray diffraction analyses revealed that the salt concentration and freezing conditions induced no structural changes in fibroin. The seeding of P19 embryonic carcinoma cells showed that the presence of salt and freezing conditions influenced the cell distribution into the scaffolds, with salt addition increasing the access of cells to deeper regions.