Osteoclastic resorption of equine cranial and postcranial bone in vitro
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- 作者：Virginia J. Kingsmill ; Colin Gray and Alan Boyde
- 关键词：Key words ; cranial ; postcranial ; bone grafts ; resorption ; osteoclasts
- 刊名：Journal of Bone and Mineral Metabolism
- 出版时间：April 2000
- 出版年：2000
- 期刊代码：115_0914-8779
- 类别：med
- 卷：18
- 期：3
- 页码：148-152
- 数据来源：sp
摘要
To address possible differences in the resorbability of cranial and postcranial bone, slices of equine frontal bone and leg (first phalanx or third metacarpus) were seeded with embryonic chick bone cells and cultured for 20–24 h. After removing the cells and drying the specimens, the areas and volumes of more than 800 resorption pits in each set were measured using a video–rate reflection confocal microscope system. Relative mineralization densities were determined by quantitative electron backscattering analysis. The mean mineralization density was greater in the leg bone, but the mean depths for resorption pits in frontal bone were smaller (median volume/area ratios, experiment 1 and experiment 2: 1.98 μm frontal and 3.79 μm leg versus 2.70 μm and 4.20 μm, respectively; P < 0.0001, Mann–Whitney), even though the areas were greater in the frontal (medians, 286 μm2 and 324 μm2, versus 242 μm2 and 201 μm2; P < 0.0001). This study has shown a difference between cranial and postcranial equine bone in the shape and size of resorption pits formed in vitro. Overall, it has shown that cranial bone may be resorbed at least as readily as postcranial bone. This result is counter to the clinical impression that cranial bone has a greater staying power than postcranial bone when used as a grafting material.