Rpc19 and Rpc40, two a-like subunits shared by nuclear RNA polymerases I and III, are interchangeable between the fission and budding yeasts
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- 作者：George V. Shpakovski and Elena K. Shematorova
- 关键词：Key wordsSchizosaccharomyces pombe ; Nuclear RNA polymerases I and III ; rpc19+ and rpc40+ genes ; α ; motif
- 刊名：Current Genetics
- 出版时间：October 1999
- 出版年：1999
- 期刊代码：63_0172-8083
- 类别：bio
- 卷：36
- 期：4
- 页码：208-214
- 数据来源：sp
摘要
The cDNAs and genes encoding the common subunits Rpc19 and Rpc40 of nuclear RNA polymerases I and III of Schizosaccharomyces pombe were isolated from cDNA and genomic libraries of the fission yeast and tested for their ability to substitute for the homologous genes in Saccharomyces cerevisiae by heterospecific complementation of corresponding null alleles and temperature-sensitive mutations. The results obtained indicate that both Sz. pombe genes (rpc19 + and rpc40 + ) are able to replace their S. cerevisiae counterparts in vivo. The primary structure and general organization of both genes were established: rpc40 + is an intronless gene, while rpc19 + contains three introns (73, 48 and 77 bp long); rpc19 + is situated on the long arm of chromosome I and rpc40 + on the long arm of chromosome II.