Evaluation of different culture conditions of Clostridium bifermentans DPH-1 for cost effective PCE degradation

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• 作者：Afroze Syeda Humayra (1)
  Yuki Hasegawa (2)
  Izumi Nomura (2)
  Young C. Chang (1)
  Takeshi Sato (3)
  Kazuhiro Takamizawa (12) tak2003@cc.gifu-u.ac.jp
• 关键词：culture conditions ; PCE dechlorination ; Clostridium bifermentans DPH ; 1 ; molasses ; bioremediation
• 刊名：Biotechnology and Bioprocess Engineering
• 出版时间：February 2005
• 出版年：2005
• 期刊代码：pc319_1226-8372
• 类别：ch
• 卷：10
• 期：1
• 页码：40-46
• 数据来源：sp

摘要

Clostridium bifermentans strain DPH-1 has already been found to dechlorinate perchloroethylene (PCE) tocis-dichloroethylene (cis-DCE)via trichloroethylene (TCE). In this study, our investigation on different culture conditions of this DPH-1 strain was extended to find a more efficient and cost effective growth medium composition for this DPH-1 strain in bioremediation practices. Temperature dependency of strain DPH-1 showed that the growth starting time and PCE degradation at 15掳C was very slow compared to that of 30掳C, but complete PCE degradation occurred in both cases. For the proper utilization of strain DPH-1 in more cost effective bioremediation practices, a simpler composition of an effective media was studied. One component of the culture medium, yeast extract, had been substituted by molasses, which served as a good source of electron donor. The DPH-1 strain in the medium containing molasses, in the presence of K₂HPO₄ and KH₂PO₄, showed identical bacterial multiplication (0.135 mg protein mL−1h−1) and PCE degradation rates (0.38 μM/h) to those of the yeast extract containing medium.