Selective isolation of bacteria from soil with hydrophobic materials
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- 作者：Shota Oku (1)
Masaya Nishiyama (1) m-248ma@nagasaki-u.ac.jp
Yuji Takao (1) - 关键词：Burkholderia cepacia – ; Burkholderia fungorum – ; Cell surface hydrophobicity – ; Octadecylsilyl silica gel – ; Polytetrafluoroethylene – ; Ralstonia pickettii – ; Selective isolation – ; Styrene– ; divinyl benzene
- 刊名：World Journal of Microbiology and Biotechnology
- 出版时间：August 2011
- 出版年：2011
- 期刊代码：121_09593993
- 类别：bio
- 卷：27
- 期：8
- 页码：1941-1945
- 数据来源：sp
摘要
Bacterial strains having a hydrophobic cell surface have often been considered as degraders of hydrophobic organic pollutants in soil. In this study, bacterial strains were isolated using hydrophobic materials from 12 soil samples, and their cell surface hydrophobicity was determined by evaluating their adherence to n-hexane. Bacterial strains isolated using polytetrafluoroethylene (PTFE) membrane were more hydrophobic on an average than those isolated with styrene–divinylbenzene (DVB) particles or octadecylsilyl silica gel (ODS) particles. Strains closely related to Burkholderia cepacia could be selectively isolated using the PTFE membrane; those closely related to Ralstonia pickettii, using ODS and DVB particles; and those closely related to B. fungorum, using DVB. These results indicate that bacterial strains having a hydrophobic cell surface or within certain phyla can be selectively isolated from soils using hydrophobic materials, and that this isolation method would be useful for collecting candidates for bioremediation of hydrophobic pollutants.