Binding of the V proteins to the nucleocapsid proteins of human parainfluenza type 2 virus
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- 作者：Noriko Watanabe ; Mitsuo Kawano ; Masato Tsurudome ; Machiko Nishio ; Morihiro Ito ; Shinji Ohgimoto ; Shigeru Suga ; Hiroshi Komada and Y. Ito
- 关键词：Key words Parainfluenza type 2 virus ; NP proteins ; Inclusion body
- 刊名：Medical Microbiology and Immunology
- 出版时间：September 1996
- 出版年：1996
- 期刊代码：168_0300-8584
- 类别：bio
- 卷：185
- 期：2
- 页码：89-94
- 数据来源：sp
摘要
Interaction of the nucleocapsid (NP) and V proteins of human parainfluenza type 2 virus (HPIV-2) was investigated using a transient expression system. When the NP proteins were co-expressed with the V proteins, some of the NP proteins were translocated into the nuclei. These findings suggest that the NP protein interact with the V proteins. We examined the interaction of the NP proteins and the P, V proteins or deletion mutants of V protein using immunofluorescence and co-immunoprecipitation plus Western blotting analyses, and showed that the V proteins of HPIV-2 bind to the NP proteins and that the N-terminal domain of V protein interacts directly with the NP proteins. When the NP proteins were co-expressed with the V proteins or the N-terminal fragments (aa 1–46), the NP proteins were detected diffusely in the nuclei of the transfected cells, and were also detected in cytoplasmic inclusions. The NP and V proteins were co-localized in the nuclei or cytoplasm. Futhermore, the NP proteins were co-precipitated with the P, V, and V(1–164) proteins by a specific antibody. The P proteins interact more closely with the NP proteins than do the V proteins. These findings indicate that the V proteins have the ability to bind the NP proteins.