Development and identification of a wheat-Psathyrostachys huashanica addition line carrying HMW-GS, LMW-GS and gliadin genes
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- 作者：Ji-Xin Zhao ; Wan-Quan Ji ; Jun Wu ; Xin-Hong Chen ; Xue-Ni Cheng ; Jian-Wei Wang ; Yu-Hui Pang ; Shu-Hui Liu and Qun-Hui Yang
- 关键词：Disomic addition line ; GISH ; PAGE ; Psathyrostachys huashanica ; Triticum aestivum
- 刊名：Genetic Resources and Crop Evolution
- 出版时间：March, 2010
- 出版年：2010
- 期刊代码：57_09259864
- 类别：bio
- 卷：57
- 期：3
- 页码：387-394
- 数据来源：sp
摘要
Psathyrostachys huashanica Keng, in the family Poaceae, subtribe Triticeae is an endangered and endemic species of China. It is characterized as Shaanxi, drought tolerant and fungus and disease resistant. In this paper, a wheat (Triticum aestivum L.)—P. huashanica disomic addition line “H9021-28-5” was developed using cytogenetics methods including SDS–PAGE, A-PAGE, and GISH. These investigations revealed that the chromosome number and configuration of “H9021-28-5” were 2n = 44 = 22 II. The mitotic and meiotic GISH analysis indicated that “H9021-28-5” contained 42 wheat chromosomes and a pair of P. huashanica chromosomes. The SDS–PAGE and A-PAGE analysis showed that “H9021-28-5” carried the HMW-GS, LMW-GS and gliadin genes of P. huashanica. The results suggest that these storage protein genes of P. huashanica had been transferred into common wheat. Development of this new germplasm is significant for enriching and improving wheat resources of storage proteins genes, and for continuing to exploit the advantageous genes of P. huashanica.