Microbial transformation of ginsenoside Rb₁ by Acremonium strictum

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• 作者：Guang-Tong Chen ; Min Yang ; Yan Song ; Zhi-Qiang Lu ; Jin-Qiang Zhang ; Hui-Lian Huang ; Li-Jun Wu and De-An Guo
• 关键词：Ginsenoside Rb1 ; Biotransformation ; Acremonium strictum
• 刊名：Applied Microbiology and Biotechnology
• 出版时间：January, 2008
• 出版年：2008
• 期刊代码：18_0175-7598
• 类别：bio
• 卷：77
• 期：6
• 页码：1345-1350
• 数据来源：sp

摘要

Preparative-scale fermentation of ginsenoside Rb₁ (1) with Acremonium strictum AS 3.2058 gave three new compounds, 12β-hydroxydammar-3-one-20 (S)-O-β-d-glucopyranoside (7), 12β, 25-dihydroxydammar-(E)-20(22)-ene-3-O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranoside (8), and 12β, 20 (R), 25-trihydroxydammar-3-O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranoside (9), along with five known compounds, ginsenoside Rd (2), gypenoside XVII (3), ginsenoside Rg₃ (4), ginsenoside F₂ (5), and compound K (6). The structural elucidation of these metabolites was based primarily on one- and two-dimensional nuclear magnetic resonance and high-resolution electron spray ionization mass spectra analyses. Among these compounds, 2–6 are also the metabolites of ginsenoside Rb₁ in mammals. This result demonstrated that microbial culture parallels mammalian metabolism; therefore, A. strictum might be a useful tool for generating mammalian metabolites of related analogs of ginsenosides for complete structural identification and for further use in pharmaceutical research in this series of compounds. In addition, the biotransformation kinetics was also investigated.