Molecular characterization of eight Indian Snakehead species (Pisces: Perciformes Channidae) using RAPD markers

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• 作者：Ajaz Ali Bhat (1)
  M. A. Haniffa (1) sxccare@gmail.com
  P. R. Divya (2)
  A. Gopalakrishnan (2)
  M. James Milton (1)
  Raj Kumar (2)
  Bilal Ahmad Paray (1)
• 关键词：Pisces &#8211 ; Indian Snakeheads &#8211 ; Molecular characterization &#8211 ; RAPD
• 刊名：Molecular Biology Reports
• 出版时间：April 2012
• 出版年：2012
• 期刊代码：39_03014851
• 类别：cp
• 卷：39
• 期：4
• 页码：4267-4273
• 数据来源：sp

摘要

Murrels (Perciformes; Channidei; Channidae) are unique group of freshwater air breathing fishes having a confined distribution to African and Asian continents. The phylogenetic relationship among eight Channid species viz. Channa aurantimaculata, Channa bleheri, Channa diplogramma, Channa gachua, Channa marulius, Channa punctatus, Channa stewartii and Channa striatus were investigated using RAPD markers. Eight random oligodecamers viz. OPAC03, OPAC05, OPAC07, OPAC09, OPAC19, OPA10, OPA11 and OPA16 were used to generate the RAPD profile. Estimates of Nei’s (Genetics, 89:583–590, 1978) unbiased genetic distance (D) demonstrated sufficient genetic divergence to discriminate the samples of different species and the values ranged from 0.3292 to 0.800 The present RAPD analyses strongly substantiate the view of earlier morphological and osteological studies of Channid species, the closer association among species in “gachua” and “marulius” groups.