Comparative Assessment of Genetic Variability in the Populations of Endemic and Endangered Yellow Catfish, Horabagrus brachysoma (Teleostei: Horabagridae), Based on Allozyme, RAPD, and Microsatellite Markers

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• 作者：P. M. Abdul Muneer (14) pmamuneer@gmail.com
  A. Gopalakrishnan (1)
  K. K. Musammilu (1)
  V. S. Basheer (1)
  V. Mohindra (2)
  K. K. Lal (2)
  K. G. Padmakumar (3)
  A. G. Ponniah (2)
• 关键词：Horabagrus brachysoma &#8211 ; Allozymes &#8211 ; RAPD &#8211 ; Genetic variation &#8211 ; Microsatellites &#8211 ; PCR &#8211 ; Polymorphism
• 刊名：Biochemical Genetics
• 出版时间：April 2012
• 出版年：2012
• 期刊代码：11_00062928
• 类别：mat
• 卷：50
• 期：3-4
• 页码：192-212
• 数据来源：sp

摘要

The comparative assessment of genetic diversity using allozymes, random amplified polymorphic DNA (RAPD), and microsatellite markers was conducted in endemic and endangered yellow catfish (Horabagrus brachysoma) sampled from three locations in Western Ghats river systems of India. Among the three markers, microsatellites show more polymorphism, having 100%polymorphic loci, whereas allozymes show the least (56%). In RAPD, 60.5%of fragments were polymorphic. Observed heterozygosity and F _ST values were very high in microsatellites, compared with the other markers. Microsatellite and RAPD markers reported a higher degree of genetic differentiation than allozymes among the populations depicted by pairwise F _ST/G _ST, AMOVA, Nei’s genetic distance, and UPGMA dendrogram. The three classes of markers demonstrated striking genetic differentiation between pairs of H. brachysoma populations. The data emphasize the need for fishery management, conservation, and rehabilitation of this species.