Myocardial enzymatic activity of renin and cathepsin D before and after bilateral nephrectomy
| 详细信息 | 推荐本文 | |
- 作者：Stephen A. Katz ; John A. Opsahl and Lynn M. Forbis
- 关键词：Key words Renin ; angiotensin system – ; cardiac myocytes – ; angiotensin – ; cardiac renin
- 刊名：Basic Research in Cardiology
- 出版时间：December 2001
- 出版年：2001
- 期刊代码：20_0300-8428
- 类别：med
- 卷：96
- 期：6
- 页码：659-668
- 数据来源：sp
摘要
A local renin-angiotensin system is present within the myocardium and can play a role in the initiation and maintenance of cardiac hypertrophy. The source of myocardial renin may be direct cardiac renin gene expression, or plasma renin of renal origin. A primary indication that myocardial renin is derived from plasma renin of renal origin was from work showing that cardiac renin activity was no longer detected 30 hours after bilateral nephrectomy (BNX). However, more recent studies have been able to detect myocardial renin after BNX. We measured normal rat cardiac renin before and after 48-hour BNX using a myocardial renin assay with improved sensitivity. The myocardial renin assay was also used to assess normal rat cardiac myocyte renin levels. Since cardiac tissue contains cathepsin D, a lysosomal enzyme capable of renin-like activity, a rat cathepsin D assay was also developed to assess cathepsin D contribution to renin-like activity.