Investigating the warming and cooling rates of human cadavers by development of a gel-filled model to validate core temperature
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- 作者：M. J. Eagle ; P. Rooney and J. N. Kearney
- 关键词：Cadaver ; Tissue donation ; Warming rate ; Cooling rate ; Core temperature
- 刊名：Cell and Tissue Banking
- 出版时间：December, 2007
- 出版年：2007
- 期刊代码：50_1389-9333
- 类别：bio
- 卷：8
- 期：4
- 页码：297-302
- 数据来源：sp
摘要
Tissue Services (within NHS Blood and Transplant) plans to bring deceased donors to its state of the art retrieval suite at its new centre in Speke, Liverpool in air-conditioned transport at circa 20掳C but without dedicated active cooling. The aim of this study was to determine how quickly a refrigerated body would warm at different ambient temperatures using a gel-filled model. Two models of a human body were prepared consisting of neoprene wetsuits filled with approximately 7 or 18 l of a viscous solution, which once set has similar properties to ballistics gel. This gel consisted of 47.5%distilled water, 47.5%glycerol and 5%agar. Final “dummy” weights were 7.4 and 18.6 kg respectively, representing “virtual” weights of approximately 40 kg and 70 kg. A K-class thermocouple probe was then inserted into a “rectal” position within each model and the models were cooled to a series of different core temperatures: 5掳C, 10掳C and 15掳C and then were placed in an orbital incubator set at 20掳C or 30掳C ambient temperature. The rate of temperature increase, in the dummy, was measured, until the model’s core temperature was close to the ambient temperature. This was done in triplicate for each size model and ambient temperature. Data indicate that increase in core temperature depends on the size of the model and the initial core temperature. For an equivalent donor weight of 70 kg and background temperature of 20掳C, core temperature rises from 5掳C to 9.2掳C; 10掳C to 13.3掳C and 15掳C to 15.5掳C after 2 h. The final core temperatures after 2 h are likely to retard bacterial growth, movement or contamination during transport. Cooling rate data indicated that a 70 kg donor equivalent cooled from 37掳C to 15掳C within 6 h in a cold room at 4掳C. This work has shown that a body can be transported without refrigeration and not cause further tissue deterioration as a result.