Cloning and Sequencing of an Intracellular D(-)-3-Hydroxybutyrate Oligomer Hydrolase from Acidovorax sp. Strain SA1 and Purification of the Enzyme

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• 作者：Akinori Sugiyama ; Mari Shiraki ; Teruyuki Kobayashi ; Gakushi Morikawa ; Mizuho Yamamoto ; Michiyo Yamaoka and Terumi Saito
• 刊名：Current Microbiology
• 出版时间：August 2002
• 出版年：2002
• 期刊代码：64_0343-8651
• 类别：bio
• 卷：45
• 期：2
• 页码：123-127
• 数据来源：sp

摘要

The gene of an intracellular D(-)-3-hydroxybutyrate oligomer hydrolase (i3HBOH) was cloned and sequenced from a poly(3-hydroxybutyrate) (PHB)-degrading bacterium, Acidovorax sp. strain SA1. The i3HBOH gene has 876 nucleotides corresponding to the deduced sequence of 292 amino acids. In this amino acid sequence, the general lipase box sequence (G-X₁-S-X₂-G) was found, whose serine residue was determined to the active sites serine by site-directed mutagenesis.