Efficient plant regeneration from cotyledon explants of bottle gourd ( Lagenaria siceraria Standl.)

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• 作者：J-S. Han ; D-G. Oh ; I-G. Mok ; H-G. Park and C. K. Kim
• 关键词：Bottle gourd (Lagenaria siceraria Standl.) ; Regeneration ; Cotyledon ; Silver nitrate ; Ploidy
• 刊名：Plant Cell Reports
• 出版时间：November 2004
• 出版年：2004
• 期刊代码：209_0721-7714
• 类别：bio
• 卷：23
• 期：5
• 页码：291-296
• 数据来源：sp

摘要

Using cotyledon explants excised from seedlings germinated in vitro, an efficient plant regeneration system via organogenesis was established for bottle gourd (Lagenaria siceraria Standl.). Maximum shoot regeneration was obtained when the proximal parts of cotyledons from 4-day-old seedlings were cultured on MS medium with 3 mg/l BA and 0.5 mg/l AgNO₃ under a 16-h photoperiod. After 3–4 weeks of culture, 21.9–80.7%of explants from the five cultivars regenerated shoots. Adventitious shoots were successfully rooted on a half-strength MS medium with 0.1 mg/l IAA for 2–3 weeks. Flow cytometric analysis revealed that most of the regenerated plants derived from culture on medium with AgNO₃ were diploid.