Structural analysis of polysaccharides from Sargassum fusiforme
- 作者：Weihua JinJing WangWenjing ZhangSumei RenNi SongQuanbin Zhang
- 会议时间：2013-10-18
- 关键词：ESI-CID-MS/MS ; laminarin ; Sargassum fusiforme ; NMR
- 作者单位：Weihua Jin(Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,PR China;University of Chinese Academy of Sciences,Beijing 100049,PR China;Nantong Branch,Institute of Oceanology,Chinese Academy of Sciences,Jiangsu 226006,PR China)Jing Wang(Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,PR China;Nantong Branch,Institute of Oceanology,Chinese Academy of Sciences,Jiangsu 226006,PR China)Wenjing Zhang(Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,PR China;University of Chinese Academy of Sciences,Beijing 100049,PR China)Sumei Ren,Ni Song(College of Medicine and Pharmaceutics,Ocean University of China,Qingdao 266003,China)Quanbin Zhang(Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,PR China)
- 母体文献：中国药学会海洋药物专业委员会第十一届海洋药物学术年会论文集
- 会议名称：中国药学会海洋药物专业委员会第十一届海洋药物学术年会
- 会议地点：海口
- 主办单位：中国药学会
- 语种：chi
摘要
Crude polysaccharide (HFS) was extracted by 0.1M HC1 firom Sargassum fusiforme (Hizikia fusiforme).Then it was firactionated by anion-exchange chromatography into three fractions HFS-1, HFS-2 and HFS-3, respectively.Chemical analysis indicated that HFS-1 was laminarin,-HFS-2 was the mixture of alginate and sulfated heteropolysaccharides and HFS-3 was mainly sulfated galactofucan.It was concluded that HFS-1 was a soluble laminarin with chains that are terminatedbyβ-D-glucose residues by NMR and 2D NMR spectra.However, -HFS-2 was still complex after the most alginate was removed.And HFS-3 might contain 3-1inked fucan sulfated at C-2, 6-1inked galactan sulfated C-2 and branched at C-4 by Fuc or 2-sulfated Fuc, galactofucan with a backbone of alternating Gal and Fuc sulfated at C-2 or alternating (Gal)n and (Fuc)n sulfated at C-2.In addition, HFS-3 still had some fucoglucuronomannan and xylan.