一水肌酸对杜浙猪肉质性状、肌肉磷酸原代谢、纤维类型特征及蛋白质溶解度的影响
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摘要
本文旨在研究饲粮添加一水肌酸(CMH)对杜浙猪肉质性状、肌肉磷酸原代谢、纤维类型特征及蛋白质溶解性的影响。试验选取体重70 kg左右杜浙猪30头,随机分为2组,每组5个重复,每个重复3头猪。对照组饲喂基础饲粮,试验组饲喂基础饲粮+0.5%CMH,试验期30 d。结果显示:与对照组相比,饲粮添加CMH对杜浙猪生长性能无显著影响(P>0.05),使背最长肌宰后24 h p H和剪切力显著增加(P<0.05),滴水损失和压榨损失显著降低(P<0.05),背最长肌的肌酸(Cr)总量、磷酸肌酸(PCr)储备量、肌酸激酶(CK)活性、白蛋白及肌原纤维蛋白溶解度显著增加(P<0.05),背最长肌肌球蛋白重链(My HC)Ⅱa mRNA比例显著降低(P<0.05)。结果提示,饲粮添加CMH对杜浙猪肉质性状具有调控作用,对肌肉磷酸原代谢能力、蛋白质溶解度及肉品系水力产生显著影响。
This experiment was conducted to investigate the effects of dietary creatine monohydrate on meat quality,phosphagen metabolism,myofiber types and protein solubility in longissimus muscle of Duroc ×Zhongbai( DZ) crossed pigs. Thirty DZ crossbred pigs( about 70 kg bodyweight) were divided into two groups with five replicates per group and three pigs per replicate. Pigs in the control group were fed the basal diet,and the others were fed the basal diet + 0. 5 % creatine monohydrate( CM H). The experiment lasted for30 days. The results showed that compared with the control group,dietary CM H had no influence on growth performance of DZ crossed pigs( P > 0. 05),the p H at 24 h postmortem and shear force of longissimus muscle were significantly increased( P < 0. 05),the drip loss and milling loss of longissimus muscle were significantly decreased( P < 0. 05),the creatine( Cr) and phosphocreatine( PCr) content,creatine kinase( CK) activity,total protein and myofibrilla protein solubilities of longissimus muscle were significantly increased( P < 0. 05),the myosin heavy chain( M y HC) Ⅱa mRNA ratio of longissimus muscle was significantly decreased( P < 0.05). In conclusion,dietary CM H can regulate meat quality,and it has significant effect on phosphagen metabolism capacity,protein solubility and water hold capacity of longissimus muscle.
This experiment was conducted to investigate the effects of dietary creatine monohydrate on meat quality,phosphagen metabolism,myofiber types and protein solubility in longissimus muscle of Duroc ×Zhongbai( DZ) crossed pigs. Thirty DZ crossbred pigs( about 70 kg bodyweight) were divided into two groups with five replicates per group and three pigs per replicate. Pigs in the control group were fed the basal diet,and the others were fed the basal diet + 0. 5 % creatine monohydrate( CM H). The experiment lasted for30 days. The results showed that compared with the control group,dietary CM H had no influence on growth performance of DZ crossed pigs( P > 0. 05),the p H at 24 h postmortem and shear force of longissimus muscle were significantly increased( P < 0. 05),the drip loss and milling loss of longissimus muscle were significantly decreased( P < 0. 05),the creatine( Cr) and phosphocreatine( PCr) content,creatine kinase( CK) activity,total protein and myofibrilla protein solubilities of longissimus muscle were significantly increased( P < 0. 05),the myosin heavy chain( M y HC) Ⅱa mRNA ratio of longissimus muscle was significantly decreased( P < 0.05). In conclusion,dietary CM H can regulate meat quality,and it has significant effect on phosphagen metabolism capacity,protein solubility and water hold capacity of longissimus muscle.
引文
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[4]YILDIZ A,OZDEMIR E,GULTURK SERDAL S.The effects of creatine long-term supplem entation on m uscle m orphology and swim m ing perform ance in rats[J].Journal of Sports Science and Medicine,2009,8(4):516-522.
[5]AZIZI M.The effect of a short-term creatine supplem entation on som e of the anaerobic perform ance and sprint swim m ing records of fem ale com petitive swimm ers[J].Procedia-Social and Behavioral Sciences,2011,15:1626-1629.
[6]ATASHAK S,JAFARI A.Effect of short-term creatine m onohydrate supplem entation on indirect m arkers of cellular dam age in young soccer players[J].Science&Sports,2012,27(2):88-93.
[7]VATANI D S,FAEAJI H,SOORI R,et al.The effects of creatine supplem entation on perform ance and horm onal response in am ateur swim m ers[J].Science&Sports,2011,26(5):272-277.
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[9]CHEN J,HUANG J Z,DEN J,et al.Use of comparative proteomics to identify the effects of creatine pyruvate on lipid and protein metabolism in broiler chickens[J].The Veterinary Journal,2012,193(2):514-521.
[10]CHEN J,MA H T,WANG M,et al.Creatine pyruvate enhances lipolysis and protein synthesis in broiler chicken[J].Agricultural Sciences in China,2011,10(12):1977-1985.
[11]程灵豪,高峰,温若竹,等.一水肌酸对肉鸭胴体组成及肌肉系水力的影响[J].浙江大学学报:农业与生命科学版,2010,36(6):635-640.
[12]SCHEFLER T L,ROSSER A L,KASTEN S C,et al.Use of dietary supplem entation withβ-guanidinopropionic acid to alter the m uscle phosphagen system,postm ortem m etabolism,and pork quality[J].M eat Science,2013,95(2):264-271.
[13]吴娟,程灵豪,高峰,等.一水肌酸对肉鸭胴体组成及肉品质的影响[J].南京农业大学学报,2011,34(4):100-104.
[14]YOUNG J F,BERTRAM H C,ROSENVOLD K,et al.Dietary creatine m onohydrate affects quality attributes of Duroc but not Landrace pork[J].M eat Science,2005,70(4):717-725.
[15]ROSENVOLD K,BERTRAM H C,YOUNG J F.Dietary creatine m onohydrate has no effect on pork quality of Danish crossbred pigs[J].M eat Science,2007,76(1):160-164.
[16]BERG E P,STAH C A,SHANNON M S,et al.The influence of dietary protein on m arket barrows and gilts supplem ented creatine m onohydrate in conjunction with a high glycem ic carbohydrate[J].M eat Science,2011,88(3):429-433.
[17]门小明.肌肉纤维类型组成对猪肉品质的影响及其机理研究[D].博士学位论文.无锡:江南大学,2007:32-39.
[18]LIU J,RUUSUNEN M,PUOLANNE E,et al.Effect of pre-rigor tem perature incubation on sarcoplasm ic protein solubility,calpain activity and m eat properties in porcine m uscle[J].LWT-Food Science and Technology,2014,55(2):483-489.
[19]TANABE R,MUROYA S,CHIKUNI K.Expression of m yosin heavy chain isoform s in porcine m uscle determ ined by m ultiplex PCR[J].Journal of Food Science,1999,64(2):222-225.
[20]呼红梅,朱荣生,张印,等.莱芜猪和杜洛克猪背最长肌肌球蛋白重链组成[J].中国农业科学,2008,41(11):3754-3759.
[21]门小明,邓波,徐子伟,等.猪宰后肌肉非乳酸供能特点及其与肉质性状相关性[J].中国农业科学,2011,44(7):1457-1465.
[22]YOUNG J F,BERTRAM H C,THEIL P K,et al.In vitro and in vivo studies of creatine m onohydrate supplem entation to Duroc and Landrace pigs[J].M eat Science,2007,76(2):342-351.
[23]CARDUCCI C,CARDUCCI C,SANTAGATA S,et al.In vitro study of uptake and synthesis of creatine and its precursors by cerebellar granule cells and astrocytes suggests som e hypotheses on the physiopathology of the inherited disorders of creatine m etabolism[J].BMC Neuroscience,2012,13:41.
[24]MILLER D W,AUGUSTINE S,ROBINSON D H,et al.Oral bioavailability of creatine supplem ents:insights into m echanism and im plications for im proved absorption[M]//BAGCHI D,NAIR S,SEN C K.Nutrition and enhanced sports perform ance:m uscle building,endurance,and strength.NewYork:Academic Press,2013:395-403.
[25]袁建琴,刘承宜,徐晓阳.骨骼肌肌酸转运及其分子机制[J].中国运动医学杂志,2009,29(3):345-348.
[26]EGAN B,ZIERATH J R.Exercise metabolism and the m olecular regulation of skeletal m uscle adaptation[J].Cell Metabolism,2013,17(2):162-184.
[27]夏伟光,左建军,冯定远.肉鸡饲粮中添加一水肌酸对骨骼肌肌酸吸收和代谢的影响[J].动物营养学报,2013,25(2):372-381.
[28]BREWER M S.Chemical and physical characteristics of m eat:water-holding capacity[M]//DEVINE C,DIKEM AN M.Encyclopedia of M eat Sciences.2nd ed.NewYork:Academ ic Press,2014:274-282.
[29]LEFAUCHEUR L.A second look into fibre typingRelation to m eat quality[J].M eat Science,2010,84(2):257-270.
[30]KIM G D,RYU Y C,JO C,et al.The characteristics of m yosin heavy chain-based fiber types in porcine longissimus dorsi m uscle[J].M eat Science,2014,96(2):712-718.
[31]GUO Y Y,LI J,SHANG J,et al.Development of m uscle-related genes and their effects on m eat quality[J].Energy Procedia,2012,16:229-233.
[32]BALDWIN K M,HADDAD F.Effects of different activity and inactivity paradigm s on m yosin heavy chain gene expression in striated m uscle[J].Journal of Applied Physiology,2001,90(1):345-357.
[33]BRAULT J J,TERJUNG R L.Creatine uptake and creatine transporter expression am ong rat skeletal m uscle fiber types[J].Am erican Journal of PhysiologyCell Physiology,2003,284(6):C1481-C1489.
[34]韩剑众,顾振宇,吴劲松,等.一水肌酸对肥育猪胴体组成和肉质的影响及机理研究[J].中国粮油学报,2007,22(3):101-106.
[35]PEARCE K L,ROSENVOLD K,ANDERSEN H J,et al.Water distribution and m obility in m eat during the conversion of m uscle to m eat and ageing and the impacts on fresh m eat quality attributes—a review[J].M eat Science,2011,89(2):111-124.
[36]CHOI Y M,LEE S H,CHOE J H,et al.Protein solubility is related to m yosin isoform s,m uscle fiber types,m eat quality traits,and postm ortem protein changes in porcine longissimus dorsi m uscle[J].Livestock Science,2010,127(2/3):183-191.
[37]CHOI Y M,RYU Y C,KIM B C.Influence of myosin heavy-and light chain isoform s on early postm ortem glycolytic rate and pork quality[J].M eat Science,2007,76(2):281-288.
[38]WARNER R.Measurement of meat quality of waterholding capacity and color:objective and subjective[M]//DEVINE C,DIKEMAN M.Encyclopedia of M eat Sciences.2nd ed.NewYork:Academ ic Press,2014:164-171.
[39]XIONG Y L.Chemical and physical characteristics of m eat[M]//DEVINE C,DIKEM AN M.Encyclopedia of m eat sciences.2nd ed.NewYork:Academ ic Press,2014:267-273.
[2]HARRIS R C,SDERLUND K,HULTMAN E.Elevation of creatine in resting and exercised m uscle of norm al subjects by creatine supplem entation[J].Clinical Science,1992,83(3):367-374.
[3]KRAEMER W J,LUK H Y,LOMBARD J R,et al.Physiological basis for creatine supplem entation in skeletal m uscle[M]//BAGCHI D,NAIR S,SEN C K.Nutrition and enhanced sports perform ance:m uscle building,endurance,and strength.NewYork:Academic Press,2013:385-394.
[4]YILDIZ A,OZDEMIR E,GULTURK SERDAL S.The effects of creatine long-term supplem entation on m uscle m orphology and swim m ing perform ance in rats[J].Journal of Sports Science and Medicine,2009,8(4):516-522.
[5]AZIZI M.The effect of a short-term creatine supplem entation on som e of the anaerobic perform ance and sprint swim m ing records of fem ale com petitive swimm ers[J].Procedia-Social and Behavioral Sciences,2011,15:1626-1629.
[6]ATASHAK S,JAFARI A.Effect of short-term creatine m onohydrate supplem entation on indirect m arkers of cellular dam age in young soccer players[J].Science&Sports,2012,27(2):88-93.
[7]VATANI D S,FAEAJI H,SOORI R,et al.The effects of creatine supplem entation on perform ance and horm onal response in am ateur swim m ers[J].Science&Sports,2011,26(5):272-277.
[8]SCHEFFLER T L,KASTEN S C,ENGLAND E M,et al.Contribution of the phosphagen system to postm ortem m uscle m etabolism in AM P-activated protein kinaseγ3 R200Q pig longissimus m uscle[J].M eat Science,2014,96(2):876-883.
[9]CHEN J,HUANG J Z,DEN J,et al.Use of comparative proteomics to identify the effects of creatine pyruvate on lipid and protein metabolism in broiler chickens[J].The Veterinary Journal,2012,193(2):514-521.
[10]CHEN J,MA H T,WANG M,et al.Creatine pyruvate enhances lipolysis and protein synthesis in broiler chicken[J].Agricultural Sciences in China,2011,10(12):1977-1985.
[11]程灵豪,高峰,温若竹,等.一水肌酸对肉鸭胴体组成及肌肉系水力的影响[J].浙江大学学报:农业与生命科学版,2010,36(6):635-640.
[12]SCHEFLER T L,ROSSER A L,KASTEN S C,et al.Use of dietary supplem entation withβ-guanidinopropionic acid to alter the m uscle phosphagen system,postm ortem m etabolism,and pork quality[J].M eat Science,2013,95(2):264-271.
[13]吴娟,程灵豪,高峰,等.一水肌酸对肉鸭胴体组成及肉品质的影响[J].南京农业大学学报,2011,34(4):100-104.
[14]YOUNG J F,BERTRAM H C,ROSENVOLD K,et al.Dietary creatine m onohydrate affects quality attributes of Duroc but not Landrace pork[J].M eat Science,2005,70(4):717-725.
[15]ROSENVOLD K,BERTRAM H C,YOUNG J F.Dietary creatine m onohydrate has no effect on pork quality of Danish crossbred pigs[J].M eat Science,2007,76(1):160-164.
[16]BERG E P,STAH C A,SHANNON M S,et al.The influence of dietary protein on m arket barrows and gilts supplem ented creatine m onohydrate in conjunction with a high glycem ic carbohydrate[J].M eat Science,2011,88(3):429-433.
[17]门小明.肌肉纤维类型组成对猪肉品质的影响及其机理研究[D].博士学位论文.无锡:江南大学,2007:32-39.
[18]LIU J,RUUSUNEN M,PUOLANNE E,et al.Effect of pre-rigor tem perature incubation on sarcoplasm ic protein solubility,calpain activity and m eat properties in porcine m uscle[J].LWT-Food Science and Technology,2014,55(2):483-489.
[19]TANABE R,MUROYA S,CHIKUNI K.Expression of m yosin heavy chain isoform s in porcine m uscle determ ined by m ultiplex PCR[J].Journal of Food Science,1999,64(2):222-225.
[20]呼红梅,朱荣生,张印,等.莱芜猪和杜洛克猪背最长肌肌球蛋白重链组成[J].中国农业科学,2008,41(11):3754-3759.
[21]门小明,邓波,徐子伟,等.猪宰后肌肉非乳酸供能特点及其与肉质性状相关性[J].中国农业科学,2011,44(7):1457-1465.
[22]YOUNG J F,BERTRAM H C,THEIL P K,et al.In vitro and in vivo studies of creatine m onohydrate supplem entation to Duroc and Landrace pigs[J].M eat Science,2007,76(2):342-351.
[23]CARDUCCI C,CARDUCCI C,SANTAGATA S,et al.In vitro study of uptake and synthesis of creatine and its precursors by cerebellar granule cells and astrocytes suggests som e hypotheses on the physiopathology of the inherited disorders of creatine m etabolism[J].BMC Neuroscience,2012,13:41.
[24]MILLER D W,AUGUSTINE S,ROBINSON D H,et al.Oral bioavailability of creatine supplem ents:insights into m echanism and im plications for im proved absorption[M]//BAGCHI D,NAIR S,SEN C K.Nutrition and enhanced sports perform ance:m uscle building,endurance,and strength.NewYork:Academic Press,2013:395-403.
[25]袁建琴,刘承宜,徐晓阳.骨骼肌肌酸转运及其分子机制[J].中国运动医学杂志,2009,29(3):345-348.
[26]EGAN B,ZIERATH J R.Exercise metabolism and the m olecular regulation of skeletal m uscle adaptation[J].Cell Metabolism,2013,17(2):162-184.
[27]夏伟光,左建军,冯定远.肉鸡饲粮中添加一水肌酸对骨骼肌肌酸吸收和代谢的影响[J].动物营养学报,2013,25(2):372-381.
[28]BREWER M S.Chemical and physical characteristics of m eat:water-holding capacity[M]//DEVINE C,DIKEM AN M.Encyclopedia of M eat Sciences.2nd ed.NewYork:Academ ic Press,2014:274-282.
[29]LEFAUCHEUR L.A second look into fibre typingRelation to m eat quality[J].M eat Science,2010,84(2):257-270.
[30]KIM G D,RYU Y C,JO C,et al.The characteristics of m yosin heavy chain-based fiber types in porcine longissimus dorsi m uscle[J].M eat Science,2014,96(2):712-718.
[31]GUO Y Y,LI J,SHANG J,et al.Development of m uscle-related genes and their effects on m eat quality[J].Energy Procedia,2012,16:229-233.
[32]BALDWIN K M,HADDAD F.Effects of different activity and inactivity paradigm s on m yosin heavy chain gene expression in striated m uscle[J].Journal of Applied Physiology,2001,90(1):345-357.
[33]BRAULT J J,TERJUNG R L.Creatine uptake and creatine transporter expression am ong rat skeletal m uscle fiber types[J].Am erican Journal of PhysiologyCell Physiology,2003,284(6):C1481-C1489.
[34]韩剑众,顾振宇,吴劲松,等.一水肌酸对肥育猪胴体组成和肉质的影响及机理研究[J].中国粮油学报,2007,22(3):101-106.
[35]PEARCE K L,ROSENVOLD K,ANDERSEN H J,et al.Water distribution and m obility in m eat during the conversion of m uscle to m eat and ageing and the impacts on fresh m eat quality attributes—a review[J].M eat Science,2011,89(2):111-124.
[36]CHOI Y M,LEE S H,CHOE J H,et al.Protein solubility is related to m yosin isoform s,m uscle fiber types,m eat quality traits,and postm ortem protein changes in porcine longissimus dorsi m uscle[J].Livestock Science,2010,127(2/3):183-191.
[37]CHOI Y M,RYU Y C,KIM B C.Influence of myosin heavy-and light chain isoform s on early postm ortem glycolytic rate and pork quality[J].M eat Science,2007,76(2):281-288.
[38]WARNER R.Measurement of meat quality of waterholding capacity and color:objective and subjective[M]//DEVINE C,DIKEMAN M.Encyclopedia of M eat Sciences.2nd ed.NewYork:Academ ic Press,2014:164-171.
[39]XIONG Y L.Chemical and physical characteristics of m eat[M]//DEVINE C,DIKEM AN M.Encyclopedia of m eat sciences.2nd ed.NewYork:Academ ic Press,2014:267-273.