超声波辅助乙醇提取茶皂素工艺研究
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摘要
为优化超声波辅助乙醇提取茶皂素的工艺,探讨茶皂素作为起泡剂、表面活性剂的可行性.试验以油茶饼粕为原材料,通过控制因素变量,采用超声波辅助乙醇,以正交试验优化获得了从油茶饼粕中提取茶皂素的最佳工艺,并分析其在手工皂中所起的重要作用.结果表明,油茶茶饼中茶皂素的最佳提取工艺为:超声波功率300W、乙醇浓度65%、浸提温度75℃、浸提时间17 min、固液比1∶7、浸提次数1次.在该条件下,茶皂素的提取量到达16.72%.当茶皂素液体量为12 mL时,制得的手工皂溶解度32.10%,起泡性169 m L,含水量33.20%,去污性良好,洁后肤感清爽.本研究对于茶皂素的提取以及作为起泡剂和表面活性剂等的应用具有一定的借鉴意义.
In order to optimize the extraction process of the tea saponin by using ultrasonic assisted ethanol, and explore the feasibility of the tea saponin as foaming agent or surfactant, the experiment used oil tea cake as raw material. The optimum extraction process of tea saponin was optimized by using ultrasonic assisted ethanol and orthogonal test, and the affecting factors of the extraction and its role in the soap production were analyzed. The research showed that the tea saponin reached its highest yield(16.72%) when the ultrasonic power was 300 W, the ethanol was 65 %, the leaching temperature was 75 ℃, the extraction time was 17 min, and the feed liquid ratio was 1∶7.When 12 mL of the tea saponin was applied, the produced soap had good detergency and fresh skin feeling with a solubility of 32.10 %, a foaming property of 169 mL, and a water content of 33.20 %. The research can provide basic data for the extraction of tea saponin and its application as foaming agent or surfactant and so on.
In order to optimize the extraction process of the tea saponin by using ultrasonic assisted ethanol, and explore the feasibility of the tea saponin as foaming agent or surfactant, the experiment used oil tea cake as raw material. The optimum extraction process of tea saponin was optimized by using ultrasonic assisted ethanol and orthogonal test, and the affecting factors of the extraction and its role in the soap production were analyzed. The research showed that the tea saponin reached its highest yield(16.72%) when the ultrasonic power was 300 W, the ethanol was 65 %, the leaching temperature was 75 ℃, the extraction time was 17 min, and the feed liquid ratio was 1∶7.When 12 mL of the tea saponin was applied, the produced soap had good detergency and fresh skin feeling with a solubility of 32.10 %, a foaming property of 169 mL, and a water content of 33.20 %. The research can provide basic data for the extraction of tea saponin and its application as foaming agent or surfactant and so on.
引文
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[12] JONG D K, MUHAMMAD I K, JIN H S, et al. HPLC fractionation and pharmacological assessment of green tea seed saponins for antimicrobial,anti-angiogenic and hemolytic activities[J]. Biotechnology and Bioprocess Engineering,2015,20(6):1035-1043.
[13] WANG Y M, REN N,GARY O,etal. Anti-proliferative effect and cell cycle arrest induced by saponins extracted from tea(Camellia sinensis)flower in human ovarian cancer cells[J]. Journal of Functional Foods,2017,37(8):310-321.
[14]熊道陵,张团结,陈金洲,等.茶皂素提取及应用研究进展[J].化工进展,2015,34(40):1080-1087.
[15]李凡,代岚,陈文庆,等.新型肥皂的制备工艺研究[J].农业科技与装备,2016,8(26):37-39.
[16]国家洗涤用品质量监督检验中心.肥皂试验方法肥皂中水分和挥发物含量的测定烘箱法:QB/T 2623.4—2003[S].北京:中国轻工业出版社,2003.
[17]中国日用化学工业研究院.衣料用洗涤剂去污力及循环洗涤性能的测定:GB/T 13174—2008[S].北京:中国标准出版社,2008.
[18]唐鹏程,王文渊,朱龙军.茶粕中茶皂素提取分离工艺的研究[J].化工管理,2017(2):185-186.
[19]陈航宇,王迪,李澍,等.手工香皂制作工艺及发展前景[J].吉林医药学院学报,2016,37(3):222-225.
[20]李扬,吴雪辉,杨柳,等.油茶茶皂素的提取及其在肥皂中的应用[J].粮食与油脂,2016,29(10):51-53.
[2]任慧璟.茶皂素的提取纯化及在日化产品中的应用[D].上海:上海海洋大学,2016:35-40.
[3]李杨.茶皂素的提取及其洗涤产品的开发研究[D].广州:华南农业大学,2016:188-193.
[4]邓桂兰,彭超英,卢峰.油茶饼粕的综合利用研究[J].广州食品工业科技,2004,20(3):130-132.
[5]国家统计局.中国统计摘要[M].北京:中国统计出版社,2010:124:145-151.
[6]熊道陵,张团结,陈金洲,等.茶皂素提取及应用研究进展[J].化工进展,2015,34(40):1080-1087.
[7]胡平平,李加兴,李忠海,等.油茶饼粕茶皂素与多糖综合提取工艺[J].食品科技,2012,37(2):196-204.
[8]邓桂兰,彭超英,卢峰.油茶饼粕的综合利用研究[J].广州食品工业科技,2004,20(3):30-35.
[9]李永峰,简健明,王少纯,等.超声波细胞粉碎辅助乙醇浸提法提取茶皂素[J].日用化学品科学,2016,39(11):17-19,25.
[10] MAO H L, WANG J K, ZHOU Y Y, et al. Effects of addition of teasaponins and soybean oil on methane production, fermentation and microbial population in the rumen of growing lambs[J]. Livestock Science,2010,129(1):56-62.
[11] DU Z X, ZHANG C J, WAN D J. Optimization of extraction process of teasaponin by ultrasonic-assisted ethanol method[J].Chemistry&Bioengineering,2015,32(3):56-59.
[12] JONG D K, MUHAMMAD I K, JIN H S, et al. HPLC fractionation and pharmacological assessment of green tea seed saponins for antimicrobial,anti-angiogenic and hemolytic activities[J]. Biotechnology and Bioprocess Engineering,2015,20(6):1035-1043.
[13] WANG Y M, REN N,GARY O,etal. Anti-proliferative effect and cell cycle arrest induced by saponins extracted from tea(Camellia sinensis)flower in human ovarian cancer cells[J]. Journal of Functional Foods,2017,37(8):310-321.
[14]熊道陵,张团结,陈金洲,等.茶皂素提取及应用研究进展[J].化工进展,2015,34(40):1080-1087.
[15]李凡,代岚,陈文庆,等.新型肥皂的制备工艺研究[J].农业科技与装备,2016,8(26):37-39.
[16]国家洗涤用品质量监督检验中心.肥皂试验方法肥皂中水分和挥发物含量的测定烘箱法:QB/T 2623.4—2003[S].北京:中国轻工业出版社,2003.
[17]中国日用化学工业研究院.衣料用洗涤剂去污力及循环洗涤性能的测定:GB/T 13174—2008[S].北京:中国标准出版社,2008.
[18]唐鹏程,王文渊,朱龙军.茶粕中茶皂素提取分离工艺的研究[J].化工管理,2017(2):185-186.
[19]陈航宇,王迪,李澍,等.手工香皂制作工艺及发展前景[J].吉林医药学院学报,2016,37(3):222-225.
[20]李扬,吴雪辉,杨柳,等.油茶茶皂素的提取及其在肥皂中的应用[J].粮食与油脂,2016,29(10):51-53.