铜试剂测定溶液铜离子浓度分光光度法的改进
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摘要
通过在显色反应体系中加大乙醇的使用量,改进了二乙基二硫代氨基甲酸钠测定溶液Cu~(2+)溶度的方法,使用NH3-NH4Cl缓冲溶液保证了反应体系稳定在pH=8~10,铜试剂与Cu2+完全显色的摩尔比为3∶1,显色反应生成的橘黄色物质稳定均一的存在于反应体系中,不需要萃取,可直接进行分光光度法的检测,在波长λ=452nm处的有效地检测范围为0.011~0.071mmol·L-1,符合朗伯-比尔定律,线性相关系数是0.9993。以柠檬酸铵作为掩蔽剂,杂质金属离子在一定浓度范围内的干扰可以得到消除。该方法避免了使用CCl4等有毒萃取溶剂,也简化了操作步骤,提高了测定的准确度,使得检测方法更加绿色快捷高效。
By increasing the amount of ethanol used in the color reaction system, the method for determining the solubility of copper ions in solution by sodium diethyldithiocarbamate was improved. The NH_3-NH_4 Cl buffer solution was used to ensure the stability of the reaction system at pH=8~10. The molar ratio of the copper reagent to the complete coloration of the copper ion is 3∶1, and the orange substance formed by the color reaction is stable and uniform in the reaction system, and does not require extraction, and can be directly detected by spectrophotometry at the wavelength λ=452 nm. The effective detection range is 0.011~0.071 mmol·L~(-1), which is in accordance with Lambert-Beer law, and the linear correlation coefficient is 0.9993. With ammonium citrate as a masking agent,interference of impurity metal ions in a certain concentration range can be eliminated. The method avoids the use of toxic extraction solvent such as CCl4, simplifies the operation steps, improves the accuracy of the measurement, and makes the detection method greener, faster and more efficient.
By increasing the amount of ethanol used in the color reaction system, the method for determining the solubility of copper ions in solution by sodium diethyldithiocarbamate was improved. The NH_3-NH_4 Cl buffer solution was used to ensure the stability of the reaction system at pH=8~10. The molar ratio of the copper reagent to the complete coloration of the copper ion is 3∶1, and the orange substance formed by the color reaction is stable and uniform in the reaction system, and does not require extraction, and can be directly detected by spectrophotometry at the wavelength λ=452 nm. The effective detection range is 0.011~0.071 mmol·L~(-1), which is in accordance with Lambert-Beer law, and the linear correlation coefficient is 0.9993. With ammonium citrate as a masking agent,interference of impurity metal ions in a certain concentration range can be eliminated. The method avoids the use of toxic extraction solvent such as CCl4, simplifies the operation steps, improves the accuracy of the measurement, and makes the detection method greener, faster and more efficient.
引文
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[12] Ayman A. G, Alaa S. A. ACloud-point extraction, preconcentration and spectrophotometric determination of trace quantities of copper in food, water and biological samples[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2014,120:88-96.
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[14]沈阳市环境监测站,环境保护部.HJ485-2009水质铜的测定-二乙基二硫代氨基甲酸钠分光光度法[S].北京:中国环境科学出版社,2009.
[2] Yoshihiro M, Keiko T,Wakaba F, et al. Dietary intake of metals and risk of Parkinson's disease:A case-control study in Japan[J].Journal of the Neurological Sciences, 2011, 306:98-102.
[3] Saurabh S. D,Sushil S. K,Ganapati S. S. A thiazoloquinoxaline based"turn-on"chemodosimeter for detection of copper ions[J]. Dyes and Pigments,2017,147:393-399.
[4] Bo S,Jiamin Z,Weifeng L, et al. Naked-eye detection of copper(Ⅱ)ions by a"clickable"fluorescent sensor[J]. Sensors and Actuators B:Chemical,2017,240:560-565.
[5] Yang X P.Jia Z H,Cheng X M, et al. Synthesis of N-acetyl-L-cys-teine capped Mn:doped CdS quantum dots for quantitative detection of copper ions[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2018,199(15):455-461.
[6] Stefano C,Vincenzo M,GokCe O, et al. A lab-on-a-tip approach to make electroanalysis user-friendly and de centralized:Detection of copper ions in river water[J]. Analytica Chimica Acta,2018,1029:1-7.
[7] Zhao G T,Liang R N,Wang F F, et al. An all-solid-state potentiometric microelectrode for detection of copper in coastal sediment pore water[J]. Sensors and Actuators B:Chemical,2019,279:369-373.
[8] Xu W T,Zhu L J,Shao X L, et al. An electrochemical biosensor based on nucleic acids enzyme and nanochannels for detecting copper(II)ion[J]. Biosensors and Bioelectronics,2018,120:168-174.
[9] Puthalapattu R P,Toyoko I. Selective detection of copper ion in water by tetradentate ligand sensor[J]. Journal of the Taiwan Institute of Chemical Engineers,2017,72:194-199.
[10] Asadollah M, Masoumeh K. A simple colorimetric chemosensorwith highly performance for detection of cyanide and copper ions and its practical application in real samples(Ⅱ)ion[J]. Journal of Photochemistry&Photobiology A:Chemistry,2018,367:22-31.
[11] SAN ANDRFS M. P, MARIN M. L, VER S. Spectrophotometric determination of copper(Ⅱ),nickel(Ⅱ)and cobalt(Ⅱ)as complexes with sodium diethyldithiocarbamate in cationic micellar medium ofhexadecyltrimethylammonium salts[J].Talanta,1994,41(2):179-185.
[12] Ayman A. G, Alaa S. A. ACloud-point extraction, preconcentration and spectrophotometric determination of trace quantities of copper in food, water and biological samples[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2014,120:88-96.
[13] Wen T,Qu F,Li N H, et al. A facile, sensitive, and rapid spectrophotometric method for copper(Ⅱ)ion detection in aqueous media using polyethyleneimine[J]. Arabian Journal of Chemistry,2017,10:1680-1685.
[14]沈阳市环境监测站,环境保护部.HJ485-2009水质铜的测定-二乙基二硫代氨基甲酸钠分光光度法[S].北京:中国环境科学出版社,2009.