摘要
P_(1B)-ATPase是在生物中广泛存在的一类可通过水解ATP跨膜运送重金属离子的蛋白,属于P型ATPase家族的一个亚类,在植物对重金属离子的转运过程中扮演着重要角色。以拟南芥和水稻中已知的P_(1B)-ATPase蛋白为对照,对中国莲P_(1B)-ATPase蛋白亚家族成员进行序列比对、系统发育、保守基序、跨膜结构、二级结构、三级结构和亚细胞定位等生物信息学分析。结果表明,中国莲存在4个P_(1B)-ATPase蛋白,分别命名为NnHMA1、NnHMA2、NnHMA3、NnHMA4,其中NnHMA1属于Zn~(2+)/Co~(2+)/Cd~(2+)/Pb~(2+)P_(1B)-ATPase (Zn亚类),NnHMA2、NnHMA3、NnHMA4属于Cu+/Ag+P_(1B)-ATPase(Cu亚类),它们均定位于质膜上,属于跨膜蛋白,有5~8个跨膜结构,含有DKTGT、TGE、C/SPx、HP等保守基序;二级结构和三级结构在组成和结构上具有较高的相似性和保守性,以α-螺旋、无规卷曲元件为主,其他元件包括延伸链和β-转角等。研究结果展示了中国莲P_(1B)-ATPase蛋白亚家族成员的序列基本信息和结构特点,为深入研究中国莲P_(1B)-ATPase蛋白及其编码基因的功能提供了理论基础。
引文
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