1株Ⅰ类新基因型新城疫病毒的基因组特性分析
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摘要
为了研究我国新型新城疫病毒的分子流行病学特性,对2016年从主动监测中分离到的1株新出现的鸭源新城疫病毒(duck/China/NX2209/2016)进行了基因组测序和遗传进化分析。序列分析结果显示该分离株全长15 198nt,F基因ORF全长为1 662bp,共编码553个氨基酸,裂解位点处的氨基酸为:~(112) ERQERL~(117),符合低致病性新城疫病毒特征。同源性分析表明该新型毒株同北美分离株具有较高的同源性。F基因高变区进化分析结果显示,该病毒属于ClassⅠ,但不属于基因型1～10,为新型毒株。完整F基因进化树结果显示,该病毒属于基因1c亚型,与北美分离株高度同源。
In order to understand the molecular epidemiology of Newcastle disease virus in China,we sequenced and analyzed one new emerging ClassⅠ NDV(duck/China/NX2209/2016)isolated from duck in 2016 during the active surveillance.Sequence analysis revealed that genome of the ClassⅠ NDV was 15 198 nt in length.The length of complete open reading frame of the F gene was 1 662 bp and encoding 553 amino acids.The putative amino acid at the cleavage site of F protein was~(112)ERQERL~(117),showed typical molecular characteristics of low pathogenic Newcastle disease virus.Homology analysis indicated the new isolate had higher homology with the NDVs isolated from North America.Genetic analysis based on the main functional region of F gene revealed that the virus was new emerging in China and didn't belong to any identified known genotype(genotype 1-10).The phylogenetic tree based on the complete F gene showed that the virus belonged to the 1cgenotype and showed highly homologous with the viruses isolated from wild birds in North American.
In order to understand the molecular epidemiology of Newcastle disease virus in China,we sequenced and analyzed one new emerging ClassⅠ NDV(duck/China/NX2209/2016)isolated from duck in 2016 during the active surveillance.Sequence analysis revealed that genome of the ClassⅠ NDV was 15 198 nt in length.The length of complete open reading frame of the F gene was 1 662 bp and encoding 553 amino acids.The putative amino acid at the cleavage site of F protein was~(112)ERQERL~(117),showed typical molecular characteristics of low pathogenic Newcastle disease virus.Homology analysis indicated the new isolate had higher homology with the NDVs isolated from North America.Genetic analysis based on the main functional region of F gene revealed that the virus was new emerging in China and didn't belong to any identified known genotype(genotype 1-10).The phylogenetic tree based on the complete F gene showed that the virus belonged to the 1cgenotype and showed highly homologous with the viruses isolated from wild birds in North American.
引文
[1]王志亮,刘华雷.新城疫[M].北京:中国农业出版社,2012:1-73.
[2]王静静,吕艳,赵云玲,等.2011-2015年中国Ⅰ类新城疫病原学监测与遗传进化分析[J].病毒学报,2017,33(2):211-215.
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[4]刘华雷,蒋小刚,张维等.一株ClassⅠ新城疫病毒中国分离株分子特性的研究[J].中国动物检疫,2008,25(8):30-33.
[5]刘华雷,张维,胡北侠,等.中国部分地区2008年Ⅰ类新城疫病毒F基因遗传变异分析[J].病毒学报,2009,25(5):382-387.
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[13] LIU H,CHEN F,ZHAO Y,et al.Genomic characterization of the first classⅠNewcastle disease virus isolated from the mainland of China[J].Virus Genens,2010,40(3):365-371.
[14]赵明,戈胜强,王静静,等.一株Ⅶb型新城疫病毒的生物学特性研究[J].中国预防兽医学报,2016,38(11):903-906.
[15] KIM L M,KING D J,CURRY P E,et al.Phylogenetic diversity among low-virulence newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to those of poultry-origin isolates[J].J Virol,2007,81(22):12641-12653.
[16] LIU X,WANG X,WU S,et al.Surveillance for avirulent Newcastle disease viruses in domestic ducks(Anas platyrhynchos and Cairina moschata)at live bird markets in Eastern China and characterization of the viruses isolated[J].Avian Pathol,2009,38(5):377-391.
[17] LEE E K,JEON W J,KWON J H,et al.Molecular epidemiological investigation of Newcastle disease virus from domestic ducks in Korea[J].Vet Microbiol,2009,134(3/4):241-248.
[18]刘华雷,王志亮.新城疫的流行历史与现状[J].中国动物检疫,2015,32(6):1-4.
[19] SHENGQING Y,KISHIDA N,ITO H,et al.Generation of velogenic Newcastle disease viruses from a nonpathogenic waterfowl isolate by passaging in chickens[J].Virology,2002,301(2):206-211.
[20] XIE Z,XIE L,CHEN A,et al.Complete genome sequence analysis of a Newcastle disease virus isolated from a wild egret[J].J Virol,2012,86(24):13854-13855.
[21]丁佳欣,李金斗,刘亚林,等.基因Ⅱ、Ⅶ型二价新城疫病毒样颗粒的制备和鉴定[J].中国兽医学报,2018,38(10):1858-1863.
[22]郑文卿,宋敏训,李建亮,等.表达1型和3型鸭甲型肝炎病毒VP1基因重组新城疫病毒的构建及其免疫原性[J].中国兽医学报,2017,37(9):1641-1647.
[2]王静静,吕艳,赵云玲,等.2011-2015年中国Ⅰ类新城疫病原学监测与遗传进化分析[J].病毒学报,2017,33(2):211-215.
[3]DIEL D G,DA SILVA L H,LIU H,et al.Genetic diversity of avian paramyxovirus type 1:proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes[J].Infect Genet Evol,2012,12(8):1770-1779.
[4]刘华雷,蒋小刚,张维等.一株ClassⅠ新城疫病毒中国分离株分子特性的研究[J].中国动物检疫,2008,25(8):30-33.
[5]刘华雷,张维,胡北侠,等.中国部分地区2008年Ⅰ类新城疫病毒F基因遗传变异分析[J].病毒学报,2009,25(5):382-387.
[6]COLLINS M S,BASHIRUDDIN J B,ALEXANDER D J.Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicity[J].Arch Virol,1993,128(3/4):363-370.
[7]SHENGQING Y,KISHIDA N,ITO H,et al.Generation of velogenic Newcastle disease viruses from a nonpathogenic waterfowl isolate by passaging in chickens[J].Virology,2002,301(2):206-211.
[8]DE LEEUW O,KOCH Q,HARTOG L,et al.Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutininneuraminidase protein[J].J Gen Virol,2005,86(6):1759-1769.
[9]WANG J,LV Y,ZHANG Y,et al.Genomic characterizations of a Newcastle disease virus isolated from ducks in live bird markets in China[J].PLoS One,2016,11(7):e0158771.
[10] KILLIAN M L.Avian influenza virus sample types,collection,and handling[J].Methods Mol Biol,2008,436:7-12.
[11] WOOLCOCK P R.Avian influenza virus isolation and propagation in chicken eggs[J].Methods Mol Biol,2008,436:35-46.
[12]赵明,戈胜强,王静静,等.新城疫病毒DF1细胞蚀斑纯化方法的建立[J].中国畜牧兽医,2014,41(6):51-54.
[13] LIU H,CHEN F,ZHAO Y,et al.Genomic characterization of the first classⅠNewcastle disease virus isolated from the mainland of China[J].Virus Genens,2010,40(3):365-371.
[14]赵明,戈胜强,王静静,等.一株Ⅶb型新城疫病毒的生物学特性研究[J].中国预防兽医学报,2016,38(11):903-906.
[15] KIM L M,KING D J,CURRY P E,et al.Phylogenetic diversity among low-virulence newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to those of poultry-origin isolates[J].J Virol,2007,81(22):12641-12653.
[16] LIU X,WANG X,WU S,et al.Surveillance for avirulent Newcastle disease viruses in domestic ducks(Anas platyrhynchos and Cairina moschata)at live bird markets in Eastern China and characterization of the viruses isolated[J].Avian Pathol,2009,38(5):377-391.
[17] LEE E K,JEON W J,KWON J H,et al.Molecular epidemiological investigation of Newcastle disease virus from domestic ducks in Korea[J].Vet Microbiol,2009,134(3/4):241-248.
[18]刘华雷,王志亮.新城疫的流行历史与现状[J].中国动物检疫,2015,32(6):1-4.
[19] SHENGQING Y,KISHIDA N,ITO H,et al.Generation of velogenic Newcastle disease viruses from a nonpathogenic waterfowl isolate by passaging in chickens[J].Virology,2002,301(2):206-211.
[20] XIE Z,XIE L,CHEN A,et al.Complete genome sequence analysis of a Newcastle disease virus isolated from a wild egret[J].J Virol,2012,86(24):13854-13855.
[21]丁佳欣,李金斗,刘亚林,等.基因Ⅱ、Ⅶ型二价新城疫病毒样颗粒的制备和鉴定[J].中国兽医学报,2018,38(10):1858-1863.
[22]郑文卿,宋敏训,李建亮,等.表达1型和3型鸭甲型肝炎病毒VP1基因重组新城疫病毒的构建及其免疫原性[J].中国兽医学报,2017,37(9):1641-1647.