膨珊瑚离体培养及形态解剖探究
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摘要
本研究以膨珊瑚茎段为外植体,采用正交试验设计,进行芽诱导离体培养研究;并应用徒手切片技术观察同化枝的形态解剖结构,分析其对生境的适应性。研究表明:膨珊瑚茎段最佳灭菌处理为75%酒精30 s+0.1%升汞11 min,最佳外植体为半木质化茎段(上段)或顶端;芽诱导的最佳培养基为1/2 MS+6-BA1.2 mg/L+NAA 0.1 mg/L;芽诱导效果最佳为半木质化茎段(上段);芽继代增殖最佳培养基为3/4 MS+ZT1.0 mg/L+NAA 0.02 mg/L+GA3 0.3 mg/L;膨珊瑚叶片退化,茎枝具有同化功能,皮层及髓部发达,含有较大的薄壁细胞,供其贮藏营养物质与水份。
In this study, the stem segments of Euphorbia oncoclada were used as explants, and orthogonal experimental design was applied to study the in vitro culture of bud induction. The morphological and anatomical structure of the assimilating branch was observed by using the technique of freehand section, which was used to analyze the adaptability of Euphorbia oncoclada to habitats. Results showed that the best sterilization treatment for the stem segments of Euphorbia oncoclada was 75% alcohol for 30 s and 0.1% mercuric chloride for 11 min, and the best explant was semi-lignification stem upper segment or tip stem. Moreover, the best medium for bud induction was 1/2 MS+6-BA 1.2 mg/L +NAA 0.1 mg/L, the best effect of bud induction was semi-lignification stem upper segment, and the best medium for bud subculture was 3/4 MS+ZT 1.0 mg/L+NAA 0.02 mg/L+GA30.3 mg/L. The leaves of Euphorbia oncoclada have been degenerated, but its stem and branch have assimilation function, and its cortex and pith are well developed, which contain large parenchyma cells for storage of nourishment and water.
In this study, the stem segments of Euphorbia oncoclada were used as explants, and orthogonal experimental design was applied to study the in vitro culture of bud induction. The morphological and anatomical structure of the assimilating branch was observed by using the technique of freehand section, which was used to analyze the adaptability of Euphorbia oncoclada to habitats. Results showed that the best sterilization treatment for the stem segments of Euphorbia oncoclada was 75% alcohol for 30 s and 0.1% mercuric chloride for 11 min, and the best explant was semi-lignification stem upper segment or tip stem. Moreover, the best medium for bud induction was 1/2 MS+6-BA 1.2 mg/L +NAA 0.1 mg/L, the best effect of bud induction was semi-lignification stem upper segment, and the best medium for bud subculture was 3/4 MS+ZT 1.0 mg/L+NAA 0.02 mg/L+GA30.3 mg/L. The leaves of Euphorbia oncoclada have been degenerated, but its stem and branch have assimilation function, and its cortex and pith are well developed, which contain large parenchyma cells for storage of nourishment and water.
引文
Gu F.G.,2008,Studies on the tissue culture and rapid reporduction technology of several ornamental plants,Thesis for M.S.,Suzhou University,Supervisor:Wan Z.G.,pp.51-58(顾福根,2008,几种观赏植物的组织培养与快速繁殖技术研究,硕士学位论文,苏州大学,导师:万志刚,pp.51-58)
Jiang L.J.,2002,Propagation technologies and cold-resistant improvement for introduced species of Euphorbia tirucalli,Dissertation for Ph.D.,Hunan Agricultural University,Su pervisor:Zhou P.H.,and Yuan Z.K.,pp.23-25,40-53(蒋丽娟,2002,引进植物绿玉树(Euphorbia tirucalli)的繁殖技术及提高抗寒性的研究,博士学位论文,湖南农业大学,导师:周朴华,袁正科,pp.23-25,40-53)
Lin F.,Li J.Y.,Huang D.M.,Xu Y.,Li Y.J.,Li Y.X.,Ding Z.L.,and Tang F.L.,2015,Study of tissue culture and plant regeneration of Aquilaria sinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1296-1299(林妃,李敬阳,黄东梅,许奕,李羽佳,李艳霞,丁哲利,唐粉玲,2015,白木香组织培养技术及植株再生的研究,基因组学与应用生物学,34(6):1296-1299)
Liu J.,Xie W.J.,and Zhang L.,2017,Callus induction and differentiation in Pedianthus tithymaloides cv.nana by orthogona experimental design,Fenzi Zhiwu Yuzhong(Molecular Plan Breeding),15(9):3599-3605(刘洁,谢伟娇,张黎,2017,蜈蚣珊瑚愈伤诱导及其分化正交试验设计,分子植物育种15(9):3599-3605)
Liu Z.L.,2011,Study on rapid propagation of energy plant Euphorbia tirucalli,Thesis for M.S.,Guangdong Ocean Un versity,Supervisor:He J.M.,and Chen B.,pp.51-58(刘召亮,2011,能源植物绿玉树快速繁殖研究,硕士学位论文广东海洋大学,导师:何觉民,陈彪,pp.51-58)
Zhu G.F.,Lv F.B.,Chen M.L.,and Wang B.Q.,2004,Somatic embryogenesis and plantlet regeneration of Euphorbia pulcherrima,Yaredai Zhiwu Kexue(Subtropical Plant Science)33(4):37-38(朱根发,吕复兵,陈明莉,王碧青,2004,一品红体细胞胚胎发生与植株再生,亚热带植物科学,33(4):37-38)
Zhang L.L.,2012,Tissue culture system of Malus Halliana,Thesis for M.S.,Northwest A&F University,Supervisor:Guo J.Z.,pp.7-19(张玲玲,2012,垂丝海棠组织培养技术体系,硕士学位论文,西北农林科技大学,导师:郭军战,pp.7-19)
Zhang X.R.,Wu H.,and Hu Z.M.,1997,Relationship between morphology and structure of leaves of the main sand-fixed plants in mao us desert and environment,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),17(5):54-60(张晓然,吴鸿,胡正海,1997,毛乌素沙地10种重要沙生植物叶的形态结构与环境关系,西北植物学报,17(5):54-60)
Xu X.L.,Zheng X.F.,and Zhou X.Y.,2013,Study on anatomical structure of vegetative organs of Portulacaria afra,Beifang Yuanyi(Northern Horticulture),(1):62-64(徐小林,郑兴峰,周晓云,2013,绿玉树营养器官解剖结构研究,北方园艺,(1):62-64)
Jiang L.J.,2002,Propagation technologies and cold-resistant improvement for introduced species of Euphorbia tirucalli,Dissertation for Ph.D.,Hunan Agricultural University,Su pervisor:Zhou P.H.,and Yuan Z.K.,pp.23-25,40-53(蒋丽娟,2002,引进植物绿玉树(Euphorbia tirucalli)的繁殖技术及提高抗寒性的研究,博士学位论文,湖南农业大学,导师:周朴华,袁正科,pp.23-25,40-53)
Lin F.,Li J.Y.,Huang D.M.,Xu Y.,Li Y.J.,Li Y.X.,Ding Z.L.,and Tang F.L.,2015,Study of tissue culture and plant regeneration of Aquilaria sinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1296-1299(林妃,李敬阳,黄东梅,许奕,李羽佳,李艳霞,丁哲利,唐粉玲,2015,白木香组织培养技术及植株再生的研究,基因组学与应用生物学,34(6):1296-1299)
Liu J.,Xie W.J.,and Zhang L.,2017,Callus induction and differentiation in Pedianthus tithymaloides cv.nana by orthogona experimental design,Fenzi Zhiwu Yuzhong(Molecular Plan Breeding),15(9):3599-3605(刘洁,谢伟娇,张黎,2017,蜈蚣珊瑚愈伤诱导及其分化正交试验设计,分子植物育种15(9):3599-3605)
Liu Z.L.,2011,Study on rapid propagation of energy plant Euphorbia tirucalli,Thesis for M.S.,Guangdong Ocean Un versity,Supervisor:He J.M.,and Chen B.,pp.51-58(刘召亮,2011,能源植物绿玉树快速繁殖研究,硕士学位论文广东海洋大学,导师:何觉民,陈彪,pp.51-58)
Zhu G.F.,Lv F.B.,Chen M.L.,and Wang B.Q.,2004,Somatic embryogenesis and plantlet regeneration of Euphorbia pulcherrima,Yaredai Zhiwu Kexue(Subtropical Plant Science)33(4):37-38(朱根发,吕复兵,陈明莉,王碧青,2004,一品红体细胞胚胎发生与植株再生,亚热带植物科学,33(4):37-38)
Zhang L.L.,2012,Tissue culture system of Malus Halliana,Thesis for M.S.,Northwest A&F University,Supervisor:Guo J.Z.,pp.7-19(张玲玲,2012,垂丝海棠组织培养技术体系,硕士学位论文,西北农林科技大学,导师:郭军战,pp.7-19)
Zhang X.R.,Wu H.,and Hu Z.M.,1997,Relationship between morphology and structure of leaves of the main sand-fixed plants in mao us desert and environment,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),17(5):54-60(张晓然,吴鸿,胡正海,1997,毛乌素沙地10种重要沙生植物叶的形态结构与环境关系,西北植物学报,17(5):54-60)
Xu X.L.,Zheng X.F.,and Zhou X.Y.,2013,Study on anatomical structure of vegetative organs of Portulacaria afra,Beifang Yuanyi(Northern Horticulture),(1):62-64(徐小林,郑兴峰,周晓云,2013,绿玉树营养器官解剖结构研究,北方园艺,(1):62-64)