不同基因型兰花组培苗对植物生长调节剂的响应
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摘要
组织培养技术是兰花苗实现工厂化标准化生产的重要技术手段之一,利用植物生长调节剂可改善兰花组培苗品质、提高出瓶存活率。本研究以3个原生种和5个杂交F1代兰花种子为组培材料,设置4种处理(CK, 0.1 mg/L TDZ, 0.5 mg/L PP333, 0.1 mg/L TDZ+0.5 mg/L PP333),研究植物生长调节剂及组合对不同基因型兰花组培苗株型的影响,其中株高及根长做为兰花试管苗的主要株型特征。结果表明,植物生长调节剂处理与对照CK组相比显著降低了8个基因型兰花的株高,抑制根的伸长,促进茎与根的横向生长;0.1 mg/L TDZ处理下兰花品种株高与对照CK组无显著差异,但根长与对照存在极显著差异,而0.5 mg/L PP333,0.1 mg/L TDZ+0.5 mg/L PP333处理后株高及根长均缩短,与对照差异均显著;不同基因型兰花的生长对同一植物生长调节剂敏感性存在极显著差异(p<0.01);根据兰花组培苗对植物生长调节剂的响应情况,可分为3类:TDZ敏感型、PP333敏感型、TDZ+PP333敏感型。
The technology of tissue culture is one of the important technical means to realize standardized production of orchids seedlings. The use of plant growth regulator can improve the quality of orchids tissue culture seedlings and the survival rate after they come out of bottles. In this study, we chose three kinds of primary seeds and five F1 hybrids seeds of orchids as tissue culture planting materials, and designed 4 kinds of treatments(CK,0.1 mg/L TDZ, 0.5 mg/L PP333 and 0.1 mg/L TDZ+0.5 mg/L PP333). The experiment was used to study the effects of plant growth regulators and combinations on the plant-type of different genotypes orchids tissue culture seedlings. The plantlet height and root length were recorded as the main plant-type characteristics of the plantlets.The results showed that compared with control CK group, the plant height of 8 genotypes of orchids was significantly reduced, the elongation of root was inhibited, and the lateral growth of stem and root was promoted through plant growth regulator treatment; The plantlet height of orchids species under 0.1 mg/L TDZ treatment had no significant difference compared with control CK group, but the root length had extremely significant difference compared with CK group, while the plant height and root length were shortened after the treatment of 0.5 mg/L PP333 and 0.1 mg/L TDZ +0.5 mg/L PP333, which had significant difference than control group. The result indicated that the sensitivity of different orchids genotypes to plant growth regulator had significant difference(p<0.01).According to the response of orchids tissue culture seedlings to plant growth regulators, they could be divided into three categories: TDZ-sensitive, PP333-sensitive and TDZ+PP333-sensitive.
The technology of tissue culture is one of the important technical means to realize standardized production of orchids seedlings. The use of plant growth regulator can improve the quality of orchids tissue culture seedlings and the survival rate after they come out of bottles. In this study, we chose three kinds of primary seeds and five F1 hybrids seeds of orchids as tissue culture planting materials, and designed 4 kinds of treatments(CK,0.1 mg/L TDZ, 0.5 mg/L PP333 and 0.1 mg/L TDZ+0.5 mg/L PP333). The experiment was used to study the effects of plant growth regulators and combinations on the plant-type of different genotypes orchids tissue culture seedlings. The plantlet height and root length were recorded as the main plant-type characteristics of the plantlets.The results showed that compared with control CK group, the plant height of 8 genotypes of orchids was significantly reduced, the elongation of root was inhibited, and the lateral growth of stem and root was promoted through plant growth regulator treatment; The plantlet height of orchids species under 0.1 mg/L TDZ treatment had no significant difference compared with control CK group, but the root length had extremely significant difference compared with CK group, while the plant height and root length were shortened after the treatment of 0.5 mg/L PP333 and 0.1 mg/L TDZ +0.5 mg/L PP333, which had significant difference than control group. The result indicated that the sensitivity of different orchids genotypes to plant growth regulator had significant difference(p<0.01).According to the response of orchids tissue culture seedlings to plant growth regulators, they could be divided into three categories: TDZ-sensitive, PP333-sensitive and TDZ+PP333-sensitive.
引文
Ding G.,Ni Y.P.,Wang M.M.,and Cheng X.Y.,2015,Research process of medicinal Denbrodium,Hubei Nongye Kexue(Hubei Agriculture Science),54(11):2561-2563(丁鸽,倪玉蓓,王萌萌,程晓雨,2015,药用石斛研究进展,湖北农业科学,54(11):2561-2563)
Dong L.,and Li Q.,2015,Influence factors of floral bud differentiation and in vitro flowering of Dendrobium nobile'Huoniao',Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),43(1):61-66(董璐,李青,2015,春石斛试管花芽分化与开花的影响因素,东北林业大学学报,43(1):61-66)
Gu X.H.,Guo B.L.,Tian J.,Ge F.,Pan J.Q.,and Zhi H.C.,2017,Utilization of plant growth regulators in growth and cultivation of medicinal plant,Zhongguo Xiandai Zhongyao(Modern Chinese Medicine),19(2):295-305(谷小红,郭宝林,田景,葛菲,潘俊倩,植汉成,2017,植物生长调节剂在药用植物生长发育和栽培中的应用,中国现代中药,19(2):295-305)
Guo Y.P.,and Li J.R.,1995,A study of rooting mechanism of kiwi plantlets in vitro induced by paclobutrazol(PP333),Yuanyi Xuebao(Acta Horticulturae Sinica),22(2):189-190(郭延平,李嘉瑞,1995,多效唑诱导猕猴桃试管苗生根的作用机理初探,园艺学报,22(2):189-190)
Jin X.H.,and Huang L.J.,2015,Investigation of original materials of Chinese medicine,Zhongguo Zhongyao Zazhi(China Journal of Chinese Materla Medica),40(13):2475-2479(金效华,黄璐琦,2015,中国石斛类药材的原植物名实考,中国中药杂志,40(13):2475-2479)
Li H.C.,2016,Studies on anti-oxidation and anti-aging of polysaccharides from eight Dendrobium species,Thsis for M.S.,South China Agricultural University,Supervisor:Liu W.,pp.1-3(李海春,2016,八种石斛多糖抗氧化、抗衰老活性的比较研究,硕士学位论文,华南农业大学,导师:刘伟,pp.1-3)
Lin F.,Li J.Y.,Huang D.M.,Xu Y.,Li Y.J.,Li Y.X.,Ding Z.L.,and Tang F.L.,2015,Study of tissue culture and plant regeneration of Aquilaria sinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1296-1299(林妃,李敬阳,黄东梅,许奕,李羽佳,李艳霞,丁哲利,唐粉玲,2015,白木香组织培养技术及植株再生的研究,基因组学与应用生物学,34(6):1296-1299)
Xu X.F.,and Huang X.L.,2003,TDZ:An effiacious plant growth regulator,Zhiwuxue Tongbao(Chinese Bulletin of Botany),20(2):227-237(徐晓峰,黄学林,2003,TDZ:一种有效的植物生长调节剂,植物学通报,20(2):227-237)
Zeng W.D.,Lu L.Y.,Xie X.Y.,and Yan H.B.,2015,Research on somatic embryogenesis and plant regeneration of cassava,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(7):1522-1526(曾文丹,陆柳英,谢向誉,严华兵,2105,木薯体细胞胚的发生及植株再生的研究,基因组学与应用生物学,34(7):1522-1526)
Zhou Y.Q.,2015,The research of tissue culture and mutagenic effect of two orchids,Thesis for M.S.,Chinese Academy of Forestry,Supervisor:Li L.B.,pp.18-35(周亚倩,2015,两种兰花的组织培养与诱变效应的研究,硕士学位论文,中国林业科学研究院,导师:李潞滨,pp.18-35)
Zhu D.Y.,Zhang H.L.,Wang J.,Li S.S.,and Yang X.,2006,Effects of PP333on the growth of Godetia grandiflora Lindl.in vitro,Zhiwu Shenglixue Tongxun(Plant Physiology Communications),42(2):232-234(朱道圩,张会丽,王锦,理莎莎,杨宵,2006,多效唑对大花高代组培苗生长的效应,植物生理学通讯,42(2):232-234)
Dong L.,and Li Q.,2015,Influence factors of floral bud differentiation and in vitro flowering of Dendrobium nobile'Huoniao',Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),43(1):61-66(董璐,李青,2015,春石斛试管花芽分化与开花的影响因素,东北林业大学学报,43(1):61-66)
Gu X.H.,Guo B.L.,Tian J.,Ge F.,Pan J.Q.,and Zhi H.C.,2017,Utilization of plant growth regulators in growth and cultivation of medicinal plant,Zhongguo Xiandai Zhongyao(Modern Chinese Medicine),19(2):295-305(谷小红,郭宝林,田景,葛菲,潘俊倩,植汉成,2017,植物生长调节剂在药用植物生长发育和栽培中的应用,中国现代中药,19(2):295-305)
Guo Y.P.,and Li J.R.,1995,A study of rooting mechanism of kiwi plantlets in vitro induced by paclobutrazol(PP333),Yuanyi Xuebao(Acta Horticulturae Sinica),22(2):189-190(郭延平,李嘉瑞,1995,多效唑诱导猕猴桃试管苗生根的作用机理初探,园艺学报,22(2):189-190)
Jin X.H.,and Huang L.J.,2015,Investigation of original materials of Chinese medicine,Zhongguo Zhongyao Zazhi(China Journal of Chinese Materla Medica),40(13):2475-2479(金效华,黄璐琦,2015,中国石斛类药材的原植物名实考,中国中药杂志,40(13):2475-2479)
Li H.C.,2016,Studies on anti-oxidation and anti-aging of polysaccharides from eight Dendrobium species,Thsis for M.S.,South China Agricultural University,Supervisor:Liu W.,pp.1-3(李海春,2016,八种石斛多糖抗氧化、抗衰老活性的比较研究,硕士学位论文,华南农业大学,导师:刘伟,pp.1-3)
Lin F.,Li J.Y.,Huang D.M.,Xu Y.,Li Y.J.,Li Y.X.,Ding Z.L.,and Tang F.L.,2015,Study of tissue culture and plant regeneration of Aquilaria sinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1296-1299(林妃,李敬阳,黄东梅,许奕,李羽佳,李艳霞,丁哲利,唐粉玲,2015,白木香组织培养技术及植株再生的研究,基因组学与应用生物学,34(6):1296-1299)
Xu X.F.,and Huang X.L.,2003,TDZ:An effiacious plant growth regulator,Zhiwuxue Tongbao(Chinese Bulletin of Botany),20(2):227-237(徐晓峰,黄学林,2003,TDZ:一种有效的植物生长调节剂,植物学通报,20(2):227-237)
Zeng W.D.,Lu L.Y.,Xie X.Y.,and Yan H.B.,2015,Research on somatic embryogenesis and plant regeneration of cassava,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(7):1522-1526(曾文丹,陆柳英,谢向誉,严华兵,2105,木薯体细胞胚的发生及植株再生的研究,基因组学与应用生物学,34(7):1522-1526)
Zhou Y.Q.,2015,The research of tissue culture and mutagenic effect of two orchids,Thesis for M.S.,Chinese Academy of Forestry,Supervisor:Li L.B.,pp.18-35(周亚倩,2015,两种兰花的组织培养与诱变效应的研究,硕士学位论文,中国林业科学研究院,导师:李潞滨,pp.18-35)
Zhu D.Y.,Zhang H.L.,Wang J.,Li S.S.,and Yang X.,2006,Effects of PP333on the growth of Godetia grandiflora Lindl.in vitro,Zhiwu Shenglixue Tongxun(Plant Physiology Communications),42(2):232-234(朱道圩,张会丽,王锦,理莎莎,杨宵,2006,多效唑对大花高代组培苗生长的效应,植物生理学通讯,42(2):232-234)