植物激素对风信子试管苗分化的影响
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摘要
本实验选用"Fondant"、"Pink Surprise"、"China Pink"和"White Pearl"4个品种鳞片分化出脱毒试管苗的鳞片叶和小鳞茎作为外植体,探究植物激素对风信子试管苗分化的影响。实验结果表明,试管苗鳞片叶直接诱导分化不定芽的能力强于小鳞茎,前者最适宜的细胞分裂素浓度分别为:6-BA 0.5 mg/L (Fondant)、TDZ 0.1 mg/L (China Pink)、6-BA 1.0 mg/L (White Pearl)和6-BA 0.5 mg/L (Pink Surprise),后者最适宜的细胞分裂素浓度分别为:ZT 0.5 mg/L (Fondant)、TDZ 0.1 mg/L (China Pink)、TDZ 0.4 mg/L (White Pearl)和ZT1.5 mg/L (Pink Surprise);试管苗鳞片叶最适宜的植物激素配比为6-BA 0.5 mg/L+IBA 0.5 mg/L (Fondant)、6-BA 0.5 mg/L+IBA 0.5 mg/L (Pink Surprise)、6-BA 1.0 mg/L+IBA 0.5 mg/L (White Pearl);小鳞茎最适宜的植物激素浓度比例为:ZT 0.5 mg/L+NAA 0.5 mg/L (Fondant)、ZT 1.5 mg/L+NAA 0.5 mg/L (Pink Surprise)、TDZ0.4 mg/L+IBA 1.0 mg/L (White Pearl);"Fondant"和"White Pearl"的最适生根浓度为NAA 1.5 mg/L和NAA1.0 mg/L。本研究为风信子组织培养体系的优化提供了科学依据。
In this experiment, the squama leaves and bulblets of virus-free plantlets derived from 4 varieties of squama, "Fondant", "Pink Surprise", "China Pink" and "White Pearl", were used as explants to explore the effects of plant hormones on the differentiation of hyacinth plantlets in vitro. The experimental results showed that the ability of directly inducing differentiation adventitious buds from squama leaves of test-tube plantlets was stronger than that of bulblets. The most suitable cytokinin concentrations for the former were 6-BA 0.5 mg/L(Fondant),TDZ 0.1 mg/L(China Pink), 6-BA 1.0 mg/L(White Pearl), and 6-BA 0.5 mg/L(Pink Surprise). For the latter, the most appropriate cytokinin concentrations were ZT 0.5 mg/L(Fondant), TDZ 0.1 mg/L(China Pink), TDZ 0.4 mg/L(White Pearl), and ZT 1.5 mg/L(Pink Surprise). The most suitable plant hormones proportions in squama leaves of test-tube plantlets were 6-BA 0.5 mg/L+IBA 0.5 mg/L(Fondant), 6-BA 0.5 mg/L+IBA 0.5 mg/L(Pink Surprise),and 6-BA 1.0 mg/L+IBA 0.5 mg/L(White Pearl). And for the small bulbs, the most appropriate plant hormone concentration are ZT 0.5 mg/L+NAA 0.5 mg/L(Fondant), ZT 1.5 mg/L+NAA 0.5 mg/L(Pink Surprise), and TDZ 0.4 mg/L+IBA 1.0 mg/L(White Pearl). In addition, the research showed that NAA 1.5 mg/L and NAA 1.0 mg/L were the optimum auxin concentrations for "Fondant" and "White Pearl". This study might provide a scientific basis for the optimization of hyacinth tissue culture system.
In this experiment, the squama leaves and bulblets of virus-free plantlets derived from 4 varieties of squama, "Fondant", "Pink Surprise", "China Pink" and "White Pearl", were used as explants to explore the effects of plant hormones on the differentiation of hyacinth plantlets in vitro. The experimental results showed that the ability of directly inducing differentiation adventitious buds from squama leaves of test-tube plantlets was stronger than that of bulblets. The most suitable cytokinin concentrations for the former were 6-BA 0.5 mg/L(Fondant),TDZ 0.1 mg/L(China Pink), 6-BA 1.0 mg/L(White Pearl), and 6-BA 0.5 mg/L(Pink Surprise). For the latter, the most appropriate cytokinin concentrations were ZT 0.5 mg/L(Fondant), TDZ 0.1 mg/L(China Pink), TDZ 0.4 mg/L(White Pearl), and ZT 1.5 mg/L(Pink Surprise). The most suitable plant hormones proportions in squama leaves of test-tube plantlets were 6-BA 0.5 mg/L+IBA 0.5 mg/L(Fondant), 6-BA 0.5 mg/L+IBA 0.5 mg/L(Pink Surprise),and 6-BA 1.0 mg/L+IBA 0.5 mg/L(White Pearl). And for the small bulbs, the most appropriate plant hormone concentration are ZT 0.5 mg/L+NAA 0.5 mg/L(Fondant), ZT 1.5 mg/L+NAA 0.5 mg/L(Pink Surprise), and TDZ 0.4 mg/L+IBA 1.0 mg/L(White Pearl). In addition, the research showed that NAA 1.5 mg/L and NAA 1.0 mg/L were the optimum auxin concentrations for "Fondant" and "White Pearl". This study might provide a scientific basis for the optimization of hyacinth tissue culture system.
引文
Che S.Q.,and Wan g C.B.,2003,Effects of hyacinth difference explants and theirs ages on bulb induce in vitro,Shanghai Jiaotong Daxue Xuebao(Journal of Shanghai Jiaotong U-niversity),21(2):135-138(车生泉,王彩波,2003,风信子不同外植体及其年龄对试管鳞茎诱导的影响,上海交通大学学报,21(2):135-138)
Geng M.L.,Ma L.,Chang L.,and Xue J.P.,2007,Progress on research of tissue culture of Frilillaria,huaibei Meitan Shifan Xueyuan Xuebao(Journal of Huaibei Coal Industry Teachers College),28(1):38-42(耿茂林,马琳,常莉,薛建平,2007,贝母组织培养研究进展,淮北煤炭师范学院学报,28(1):38-42)
Li Y.P.,2011,Studies on the reproductive biology of Hyacinthus orientalis L.,Dissertation for Ph.D.,Nanjing Forestry University,Supervisor:Tang G.G.,pp.102(李玉萍,2011,风信子繁殖生物学研究,博士学位论文,南京林业大学,导师:汤庚国,pp.102)
Lin F.,Li J.Y.,Huang D.M.,Xu Y.,Li Y.J.,Li Y.X.,Ding Z.L.,and Tang F.L.,2015,Study of tissue culture and plant regeneration of Aquilaria sinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1296-1299(林妃,李敬阳,黄东梅,许奕,李羽佳,李艳霞,丁哲利,唐粉玲,2015,白木香组织培养技术及植株再生的研究,基因组学与应用生物学,34(6):1296-1299)
Liu S.,Sun Y.D.,and Li Y.Q.,2011,Study on the rapid propagation in vitro of common Hyacinthus,beifang yuangyi(Northern Horticulture),(20):137-139(刘爽,孙余丹,李叶青,2011,风信子离体快繁技术研究,北方园艺,(20):137-139)
Liu Y.G.,and Xu Z.Q.,2001,Tissue culture and plant regeneration of Hyacinthus orientalis L.,Xibei Daxue Xuebao(Journal of Northwest University),31(3):255-258(刘勇刚,徐子勤,2001,风信子的组织培养和植株再生,西北大学学报,31(3):255-258)
Lu W.L.,Bai S.N.,and Zhang X.S.,1999,Induction of continuous tepal differentiation from in vitro regenerated flower buds of Hyacinthus orientalis,Zhiwu Xuebao(Acta Botanica Sinica),41(9):921-926(陆文樑,白书农,张宪省,1999,诱导风信子再生花芽不断分化花被片的研究,植物学报,41(9):921-926)
Sun H.M.,Wang J.X.,Duan X.,Zhang J.,Li X.Y.,and Gao H.,2015,Establishment of an in vitro rapid propagation system in oriental lily"Double Surprise",Shenyang Nongye Daxue Xuebao(Journal of Shenyang Agricultural University),46(4):391-397(孙红梅,王锦霞,段鑫,张静,李雪艳,高鹤,2015,重瓣东方百合Double surprise离体快繁技术体系的建立,沈阳农业大学学报,46(4):391-397)
Wang Q.C.,1992,Plant hormones and adventitious root formation in cuttings,Sichuan Nongye Daxue Xuebao(Journal of Sichuan Agricultural University),10(1):33-39(王乔春,1992,植物激素与插条不定根的形成,四川农业大学学报,10(1):33-39)
Yang Z.H.,and Wang J.Y.,1987,Effects of plant growth regulator upon lily propagation by tissue culture,Xibei Nongye Daxue Xuebao(Journal of Northwest Kasetsart University),15(3):72-78(杨增海,王聚瀛,1987,植物生长调节剂对百合组织培养繁殖的效应,西北农业大学学报,15(3):72-78)
Geng M.L.,Ma L.,Chang L.,and Xue J.P.,2007,Progress on research of tissue culture of Frilillaria,huaibei Meitan Shifan Xueyuan Xuebao(Journal of Huaibei Coal Industry Teachers College),28(1):38-42(耿茂林,马琳,常莉,薛建平,2007,贝母组织培养研究进展,淮北煤炭师范学院学报,28(1):38-42)
Li Y.P.,2011,Studies on the reproductive biology of Hyacinthus orientalis L.,Dissertation for Ph.D.,Nanjing Forestry University,Supervisor:Tang G.G.,pp.102(李玉萍,2011,风信子繁殖生物学研究,博士学位论文,南京林业大学,导师:汤庚国,pp.102)
Lin F.,Li J.Y.,Huang D.M.,Xu Y.,Li Y.J.,Li Y.X.,Ding Z.L.,and Tang F.L.,2015,Study of tissue culture and plant regeneration of Aquilaria sinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1296-1299(林妃,李敬阳,黄东梅,许奕,李羽佳,李艳霞,丁哲利,唐粉玲,2015,白木香组织培养技术及植株再生的研究,基因组学与应用生物学,34(6):1296-1299)
Liu S.,Sun Y.D.,and Li Y.Q.,2011,Study on the rapid propagation in vitro of common Hyacinthus,beifang yuangyi(Northern Horticulture),(20):137-139(刘爽,孙余丹,李叶青,2011,风信子离体快繁技术研究,北方园艺,(20):137-139)
Liu Y.G.,and Xu Z.Q.,2001,Tissue culture and plant regeneration of Hyacinthus orientalis L.,Xibei Daxue Xuebao(Journal of Northwest University),31(3):255-258(刘勇刚,徐子勤,2001,风信子的组织培养和植株再生,西北大学学报,31(3):255-258)
Lu W.L.,Bai S.N.,and Zhang X.S.,1999,Induction of continuous tepal differentiation from in vitro regenerated flower buds of Hyacinthus orientalis,Zhiwu Xuebao(Acta Botanica Sinica),41(9):921-926(陆文樑,白书农,张宪省,1999,诱导风信子再生花芽不断分化花被片的研究,植物学报,41(9):921-926)
Sun H.M.,Wang J.X.,Duan X.,Zhang J.,Li X.Y.,and Gao H.,2015,Establishment of an in vitro rapid propagation system in oriental lily"Double Surprise",Shenyang Nongye Daxue Xuebao(Journal of Shenyang Agricultural University),46(4):391-397(孙红梅,王锦霞,段鑫,张静,李雪艳,高鹤,2015,重瓣东方百合Double surprise离体快繁技术体系的建立,沈阳农业大学学报,46(4):391-397)
Wang Q.C.,1992,Plant hormones and adventitious root formation in cuttings,Sichuan Nongye Daxue Xuebao(Journal of Sichuan Agricultural University),10(1):33-39(王乔春,1992,植物激素与插条不定根的形成,四川农业大学学报,10(1):33-39)
Yang Z.H.,and Wang J.Y.,1987,Effects of plant growth regulator upon lily propagation by tissue culture,Xibei Nongye Daxue Xuebao(Journal of Northwest Kasetsart University),15(3):72-78(杨增海,王聚瀛,1987,植物生长调节剂对百合组织培养繁殖的效应,西北农业大学学报,15(3):72-78)