浙皖地理种源钩锥遗传多样性分析及选育策略研究
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摘要
钩锥(Castanopsis tibetana Hance)为锥属(Castanopsis)高大乔木,具有材用、果用及观赏价值,其研究刚起步。为了制订该树种的选育策略,本研究基于钩锥同属或同科物种SSR引物,用筛选出的20对引物对‘浙皖龙泉’(LQ)及‘休宁’(XN)两个地理源钩锥半同胞家系及不同居群进行遗传多样性分析。结果发现,钩锥同属或同科物种间SSR引物的通用性较高;各遗传参数休宁的略大于龙泉,但二者差异不大;居群的遗传参数要大于半同胞家系,居群的等位基因数(Na)平均为3.85,有效等位基因数(Ne)平均为2.23,期望杂合度(He)平均为0.42,观测杂合度(Ho)平均为0.34,Shannon多样性指数(I)平均为0.79,主要变异在居群内;供试的材料无论在家系水平还是在居群水平均可按种源聚成二类。因此,该树种的选育应种源选择为先,在此基础上再基于生产目的进行优良单株的选择,同时因该树种分布范围广,因而要进行多点多年的引种对比试验。
Castanopsis tibetana is a tall tree species belonging to Castanopsis, which has the value in material,fruit and ornamentation. The study of this species has just started. In order to develop strategy for selective breeding of this species, 20 pairs of SSR primers were screened out of the SSR primers from other species which were in the same category or same family of C. tibetana. Then, the genetic diversity of C. tibetana half sib families and different populations from two geo-provenances, viz. Longquan of Zhejiang and Xiuning of Anhui, were analyzed. Results showed that the SSR primers had a high transferability between C. tibetana and other species of the same genus or same family; genetic parameters of Xiuning were a little bit higher in value than those of Longquan, but the diffrence was not significant; genetic parameters of populations were higher in value than those of families. The average values of Na, Ne, I, Ho, and He for populations were 3.85, 2.23, 0.79, 0.34, and 0.42,respectively, and main variation existed within populations; all the samples tested could be clustered into two groups based on provenances either in terms of families or populations. Therefore, the selection of provenances should be the first choice for this tree species, followed by elite individual trees for the purpose of production.Meanwhile, a test of multi-sites and comparison for introduction of this species should be performed for years because of a wide range of distribution of this species.
Castanopsis tibetana is a tall tree species belonging to Castanopsis, which has the value in material,fruit and ornamentation. The study of this species has just started. In order to develop strategy for selective breeding of this species, 20 pairs of SSR primers were screened out of the SSR primers from other species which were in the same category or same family of C. tibetana. Then, the genetic diversity of C. tibetana half sib families and different populations from two geo-provenances, viz. Longquan of Zhejiang and Xiuning of Anhui, were analyzed. Results showed that the SSR primers had a high transferability between C. tibetana and other species of the same genus or same family; genetic parameters of Xiuning were a little bit higher in value than those of Longquan, but the diffrence was not significant; genetic parameters of populations were higher in value than those of families. The average values of Na, Ne, I, Ho, and He for populations were 3.85, 2.23, 0.79, 0.34, and 0.42,respectively, and main variation existed within populations; all the samples tested could be clustered into two groups based on provenances either in terms of families or populations. Therefore, the selection of provenances should be the first choice for this tree species, followed by elite individual trees for the purpose of production.Meanwhile, a test of multi-sites and comparison for introduction of this species should be performed for years because of a wide range of distribution of this species.
引文
Cai Y.Q.,Xu D.Q.,Chen S.,Zhang B.,Wang P.L.,Zhou X.W.Li Z.H.,and Wu Y.,2016,Effects of different fertilization on growth and physiological characteristics of Castanopsi tibetana seedlings,Zhongnan Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology),36(3):33-37(蔡雅桥,许德琼,陈松,张斌,王佩兰,周鑫伟,李志辉,吴毅,2016,配方施肥对钩栗生长和生理特性的影响,中南林业科技大学学报,36(3):33-37)
Chen Y.,2007,Techniques for artificial seedling culture in Castanopsis tibetana,Jishu Kaifa(Forest Science and Tech nology),21(3):89-90(陈养,2007,钩栗人工育苗技术研究技术开发,21(3):89-90)
Editorial board of Flora of China,ed.,1998,Flora of China Science Press.,Beijing,China,pp.22-23(《中国植物志》编辑委员会,1998,中国植物志,科学出版社,中国,北京,pp22-23)
Evanno G.,Regnaut S.,and Goudet J.,2005,Detecting the numbe of clusters of individuals using the software STRUCTUREa simulation study,Molecular Ecology,14(8):2611-2620
Fu D.D.,Wang J.,Liu Y.F.,and Huang H.W.,2010,Isolation o microsatellite markers for Castanopsis fargesii(Fagaceae)Redai Yaredai Zhiwu Xuebao(Journal of Tropical and Subtropical Botany),18(5):541-546(付丹丹,王静,刘义飞,黄宏文,2010,栲树微卫星分子标记的开发,热带亚热带植物学报,18(5):541-546)
Hamrick J.L.,1987,Gene flow and distribution of genetic varia tion in plant populations,In:Urbanska K.,ed.,Differentiation patterns in higher plants Academic Press,New York,USApp.53-67
Huang G.M.,Hong L.,Ye W.H.,Shen H.,Cao H.L.,and Xiao W.,2009,Isolation and characterization of polymorphic microsatellite loci in Castanopsis chinensis Hance(Fagaceae)Conserv.Genet.,10(4):1069-1071
Huang R.L.,Wang Y.,Liu X.W.,He Y.H.,and Jiang Y.,2014,Acomparison among containerized seedlings growing in different media and treated with rooting agents in Castanopsi tibetana,Linye Shiyong Jishu(Practical Forestry Techno logy),(7):41-44(黄荣林,王勇,刘晓蔚,何应会,蒋燚2014,钩栗不同基质和生根剂处理容器育苗对比试验研究,林业实用技术,(7):41-44)
Li C.,and Sun Y.,2012,Transferability analysis of EST-SSRmarkers of Castanea mollissima to Castanopsis fargesii Guangxi Zhiwu(Guihaia),32(3):293-297(李春,孙晔,2012中国板栗EST-SSR分子标记在栲树中的通用性分析,广西植物,32(3):293-297)
Li C.J.,2012,Characteristics of Castanopsis tibetana in the mountain area of southern Anhui Province and technique for seeding and seedling culture,Xiandai Nongye Keji(Modern Agricultural Sciences and Technology),(13):185-186(李纯教,2012,皖南山区钩栲特征特性及播种育苗技术现代农业科技,(13):185-186)
Li J.,Zeng Y.R.,Shen D.F.,Xia G.H.,Huang Y.Z.,Huang Y.J.Chang J.,Huang J.Q.,and Wang Z.J.,2014,Development o SSR markers in hickory(Carya cathayensis Sarg)and thei transferability to other species of carya,Journal of Applied Statistics,15(15):357-379
Liao Y.Y.,2015,Effects of developing moso bamboo plantation on changes in the population of Castanopsis tibetana,Lvse Keji(Green Sciences and Technology),(6):39-41(廖元英2015,发展毛竹林对钩栲林种群变化的影响,绿色科技(6):39-41)
Lin M.,and Huang Z.A.,2003,Analysis of Castanopsis tibetana population life table,Fujian Linye Keji(Journal of Fujian Forestry Science and Technology),30(2):9-13(林敏,黄宗安2003,钩栗种群生命表分析,福建林业科技,30(2):9-13)
Liu H.K.,2014,SRAP Marker-based analysis of genetic diversity in Torreya grandis,Thesis for M.S.,Zhejiang A&F University,Supervisor:Zeng Y.R.,pp.4-60(刘浩凯,2014,榧树遗传多样性的SRAP标记分析,硕士学位论文,浙江农林大学,导师:曾燕如,pp.4-60)
Lv X.Y.,Zhao Y.,Zhao G.M.,Zeng Y.R.,Song X.Z.,and Yang H.,2014,Establishment of SRAP-PCR reaction system in Cinnamomum japonicum var.chenii,Zhejiang Linye Kej(Journal of Zhejiang Forestry Science and Technology),3(34):23-27(吕昕谣,赵颖,赵国淼,曾燕如,宋绪忠,杨华2014,普陀樟SRAP-PCR反应体系的建立,浙江林业科技,3(34):23-27)
Shi Y.S.,Zhang J.,Jiang K.,Cui M.Y.,and Li Y.Y.,2011Development and characterization of polymorphic microsatellite markers in Castanopsis sclerophylla(Fagaceae)American Journal of Botany,98(2):19-21
Song X.Y.,2002,Research on niche of Castanopsis tibetana population and its main associated species in Sanming Fujian,Yaredai Zhiwu Kexue(Subtropical Plant Science)(3):27-30(宋晓英,2002,福建三明钩栲种群与主要伴生树种生态位研究,亚热带植物科学,31(3):27-30)
Tian Y.L.,Li Z.H.,Yang M.H.,and Zhang B.,2015,Establishmen and optimization of Castanopsis tibetana Hance ISSR-PCRreaction system,Zhongguo Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology)35(2):32-37(田艳伶,李志辉,杨模华,张斌,2015,钩栗ISSR-PCR反应体系的建立与优化,中南林业科技大学学报,35(2):32-37)
Tian Y.L.,Li B.H.,Li Z.H.,Yang M.H.,Zhang B.,and Zhou XW.,2017,Genetic diversity analysis on five populations o Castanopsis tibetana from Fujian Province based on ISSRmarker,Guangxi Zhiwu(Guihaia),37(1):42-48(田艳伶,李柏海,李志辉,杨模华,张斌,周鑫伟,2017,基于ISSR标记的福建省钩锥遗传多样性分析,广西植物,37(1):42-48)
Ueno S.,Aoki K.,and Tsumura Y.,2009,Generation of expressed sequence tags and development of microsatellite markers fo Castanopsis sieboldii var.sieboldii(Fagaceae),Ann.For.Sci.66(5):509-509
Ueno S.,Yoshimaru H.,Kawahara T.,and Yamamoto S.,2003,Afurther six microsatellite markers for Castanopsis cuspidat var.sieboldii Nakai,Conserv.Genet.,4(6):813-815
Wang P.L.,Kang H.R.,Li B.H.,Li Z.H.,Zhang B.,and Wu Y.2016,Photosynthetic traits of the precious native species o Castanopsis tibetana seedlings among different provenances Zhongnan Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology),36(3):38-45(王佩兰,康华荣,李伯海,李志辉,张斌,吴毅,2016,乡土珍贵树种钩栗幼苗种源间光合生理特性研究,中南林业科技大学学报,36(3):38-45)
Wang P.L.,Xu D.L.,Zhang B.,Li H.,Hu J.J.,and Li Z.H.,2013Analyses of morphological character and physiologica indices of Castanopsis tibetana seed,Zhongzi(Seed),32(8)7-16(王佩兰,许德禄,张斌,李何,胡俊靖,李志辉,2013钩栗种子形态特征及主要生理指标测定分析,种子,32(8):7-10)
Wang P.L.,Xu D.L.,Zhang B.,and Li Z.H.,2014,Comparison study on seedling growth and photosynthetic characteristic of Castanopsis tibetana among different provenances,Beifang Yuanyi(Northern Horticulture),(5):20-25(王佩兰,许德禄,张斌,李志辉,2014,钩栗种源间幼苗生长状况和光合特性比较研究,北方园艺,(5):20-25)
Wang Y.,Liu X.W.,Liu X.S.,Deng Y.H.,and Jiang Y.,2016Comparison experiment of root cutting at seedling stage in different provenances of Castanopsis tibetana Hance,Beifang Yuanyi(Northern Horticulture),(4):6-11(王勇,刘晓蔚,刘雄盛,邓玉华,蒋燚,2016,钩栗不同种源间苗期切根处理比较试验,北方园艺,(4):6-11)
Wu B.H.,2015,Structural analysis on natural community o Castanopsis tibetana in Youxi Qipanding,Lvse Keji(Journa of Green Science and Technology),(7):55-58(吴碧华,2015尤溪棋盘顶钩栲天然林群落结构分析,绿色科技,(7)55-58)
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Yang J.W.,Jiang J.M.,Jia N.,Dong L.M.,Ding Y.W.,Hu C.Y.Tang Y.Y.,and Zeng Y.R.,2017,Study on variation in trait of seed and seedling and their heritability in Castanopsi tibetana,Zhejiang Linye Keji(Journal of Zhejiang Forestry Science and Technology),2(37):29-35(杨珺雯,姜俊马,贾宁,董雷鸣,丁奕炜,胡纯瑜,唐研耀,曾燕如,2017,钩锥种子及其苗木性状的遗传变异研究,浙江林业科技,2(37):29-35)
Yang X.,Liu F.,Zhang Y.,Cheng Y.F.,Xue L.B.,and Chen X.H2016,Study on the genetic diversity of eggplant germplasm with SSR markers,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(12):3450-3457(杨旭,刘飞,张宇,成玉富,薛林宝,陈学好,2016,利用SSR标记研究茄子种质资源遗传多样性,基因组学与应用生物学,35(12):3450-3457)
Ye L.J.,Wang J.,Sun P.,Dong S.P.,and Zhang Z.Y.,2014,The transferability of nuclear microsatellite markers in fou castanopsis species to Castanopsis tibetana(Fagaceae),Zhiwu Fenlei Yu Ziyuan Xuebao(Plant Diversity and Resources)36(4):443-448(叶林江,王静,孙鹏,董书鹏,张志勇,2014四种栲属植物微卫星标记在钩栲中的通用性检测,植物分类与资源学报,36(4):443-448)
Young A.,Boyle T.,and Brown T.,1996,The population genetic consequences of habitat fragmentation for plants,Trend Ecol.Evol.,11(10):413-418
Zeng J.J.,Zhang C.,Li Z.H.,Yang M.H.,Niu F.H.,and Zhang B.2016,Influence of different rooting agents and cutting treatments on the rooting of Castanopsis tibetana cuttings Zhongnan Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology),36(6):56-60(曾晶珏,张成,李志辉,杨模华,牛芳华,张斌,2016,不同生根剂及处理枝条方式对钩栗扦插生根的影响,中南林业科技大学学报,36(6):56-60)
Zhang J.S.,2005,Study of Castanopsis tibetana community dominant plant population competition,Fujian Linye Kej(Journal of Fujian Forestry Science and Technology),32(4)82-85(张嘉生,2005,钩栲群落优势植物种群竞争的研究福建林业科技,32(4):82-85)
Zheng X.X.,Feng S.P.,and Li W.G.,2009,Research progress o DNA molecular marker,Anhui Nongye Kexue(Journal o Anhui Agricultural Sciences),37(26):12420-12422(郑学项,冯素萍,李维国,2009,DNA分子标记研究进展,安徽农业科学,37(26):12420-12422)
Chen Y.,2007,Techniques for artificial seedling culture in Castanopsis tibetana,Jishu Kaifa(Forest Science and Tech nology),21(3):89-90(陈养,2007,钩栗人工育苗技术研究技术开发,21(3):89-90)
Editorial board of Flora of China,ed.,1998,Flora of China Science Press.,Beijing,China,pp.22-23(《中国植物志》编辑委员会,1998,中国植物志,科学出版社,中国,北京,pp22-23)
Evanno G.,Regnaut S.,and Goudet J.,2005,Detecting the numbe of clusters of individuals using the software STRUCTUREa simulation study,Molecular Ecology,14(8):2611-2620
Fu D.D.,Wang J.,Liu Y.F.,and Huang H.W.,2010,Isolation o microsatellite markers for Castanopsis fargesii(Fagaceae)Redai Yaredai Zhiwu Xuebao(Journal of Tropical and Subtropical Botany),18(5):541-546(付丹丹,王静,刘义飞,黄宏文,2010,栲树微卫星分子标记的开发,热带亚热带植物学报,18(5):541-546)
Hamrick J.L.,1987,Gene flow and distribution of genetic varia tion in plant populations,In:Urbanska K.,ed.,Differentiation patterns in higher plants Academic Press,New York,USApp.53-67
Huang G.M.,Hong L.,Ye W.H.,Shen H.,Cao H.L.,and Xiao W.,2009,Isolation and characterization of polymorphic microsatellite loci in Castanopsis chinensis Hance(Fagaceae)Conserv.Genet.,10(4):1069-1071
Huang R.L.,Wang Y.,Liu X.W.,He Y.H.,and Jiang Y.,2014,Acomparison among containerized seedlings growing in different media and treated with rooting agents in Castanopsi tibetana,Linye Shiyong Jishu(Practical Forestry Techno logy),(7):41-44(黄荣林,王勇,刘晓蔚,何应会,蒋燚2014,钩栗不同基质和生根剂处理容器育苗对比试验研究,林业实用技术,(7):41-44)
Li C.,and Sun Y.,2012,Transferability analysis of EST-SSRmarkers of Castanea mollissima to Castanopsis fargesii Guangxi Zhiwu(Guihaia),32(3):293-297(李春,孙晔,2012中国板栗EST-SSR分子标记在栲树中的通用性分析,广西植物,32(3):293-297)
Li C.J.,2012,Characteristics of Castanopsis tibetana in the mountain area of southern Anhui Province and technique for seeding and seedling culture,Xiandai Nongye Keji(Modern Agricultural Sciences and Technology),(13):185-186(李纯教,2012,皖南山区钩栲特征特性及播种育苗技术现代农业科技,(13):185-186)
Li J.,Zeng Y.R.,Shen D.F.,Xia G.H.,Huang Y.Z.,Huang Y.J.Chang J.,Huang J.Q.,and Wang Z.J.,2014,Development o SSR markers in hickory(Carya cathayensis Sarg)and thei transferability to other species of carya,Journal of Applied Statistics,15(15):357-379
Liao Y.Y.,2015,Effects of developing moso bamboo plantation on changes in the population of Castanopsis tibetana,Lvse Keji(Green Sciences and Technology),(6):39-41(廖元英2015,发展毛竹林对钩栲林种群变化的影响,绿色科技(6):39-41)
Lin M.,and Huang Z.A.,2003,Analysis of Castanopsis tibetana population life table,Fujian Linye Keji(Journal of Fujian Forestry Science and Technology),30(2):9-13(林敏,黄宗安2003,钩栗种群生命表分析,福建林业科技,30(2):9-13)
Liu H.K.,2014,SRAP Marker-based analysis of genetic diversity in Torreya grandis,Thesis for M.S.,Zhejiang A&F University,Supervisor:Zeng Y.R.,pp.4-60(刘浩凯,2014,榧树遗传多样性的SRAP标记分析,硕士学位论文,浙江农林大学,导师:曾燕如,pp.4-60)
Lv X.Y.,Zhao Y.,Zhao G.M.,Zeng Y.R.,Song X.Z.,and Yang H.,2014,Establishment of SRAP-PCR reaction system in Cinnamomum japonicum var.chenii,Zhejiang Linye Kej(Journal of Zhejiang Forestry Science and Technology),3(34):23-27(吕昕谣,赵颖,赵国淼,曾燕如,宋绪忠,杨华2014,普陀樟SRAP-PCR反应体系的建立,浙江林业科技,3(34):23-27)
Shi Y.S.,Zhang J.,Jiang K.,Cui M.Y.,and Li Y.Y.,2011Development and characterization of polymorphic microsatellite markers in Castanopsis sclerophylla(Fagaceae)American Journal of Botany,98(2):19-21
Song X.Y.,2002,Research on niche of Castanopsis tibetana population and its main associated species in Sanming Fujian,Yaredai Zhiwu Kexue(Subtropical Plant Science)(3):27-30(宋晓英,2002,福建三明钩栲种群与主要伴生树种生态位研究,亚热带植物科学,31(3):27-30)
Tian Y.L.,Li Z.H.,Yang M.H.,and Zhang B.,2015,Establishmen and optimization of Castanopsis tibetana Hance ISSR-PCRreaction system,Zhongguo Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology)35(2):32-37(田艳伶,李志辉,杨模华,张斌,2015,钩栗ISSR-PCR反应体系的建立与优化,中南林业科技大学学报,35(2):32-37)
Tian Y.L.,Li B.H.,Li Z.H.,Yang M.H.,Zhang B.,and Zhou XW.,2017,Genetic diversity analysis on five populations o Castanopsis tibetana from Fujian Province based on ISSRmarker,Guangxi Zhiwu(Guihaia),37(1):42-48(田艳伶,李柏海,李志辉,杨模华,张斌,周鑫伟,2017,基于ISSR标记的福建省钩锥遗传多样性分析,广西植物,37(1):42-48)
Ueno S.,Aoki K.,and Tsumura Y.,2009,Generation of expressed sequence tags and development of microsatellite markers fo Castanopsis sieboldii var.sieboldii(Fagaceae),Ann.For.Sci.66(5):509-509
Ueno S.,Yoshimaru H.,Kawahara T.,and Yamamoto S.,2003,Afurther six microsatellite markers for Castanopsis cuspidat var.sieboldii Nakai,Conserv.Genet.,4(6):813-815
Wang P.L.,Kang H.R.,Li B.H.,Li Z.H.,Zhang B.,and Wu Y.2016,Photosynthetic traits of the precious native species o Castanopsis tibetana seedlings among different provenances Zhongnan Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology),36(3):38-45(王佩兰,康华荣,李伯海,李志辉,张斌,吴毅,2016,乡土珍贵树种钩栗幼苗种源间光合生理特性研究,中南林业科技大学学报,36(3):38-45)
Wang P.L.,Xu D.L.,Zhang B.,Li H.,Hu J.J.,and Li Z.H.,2013Analyses of morphological character and physiologica indices of Castanopsis tibetana seed,Zhongzi(Seed),32(8)7-16(王佩兰,许德禄,张斌,李何,胡俊靖,李志辉,2013钩栗种子形态特征及主要生理指标测定分析,种子,32(8):7-10)
Wang P.L.,Xu D.L.,Zhang B.,and Li Z.H.,2014,Comparison study on seedling growth and photosynthetic characteristic of Castanopsis tibetana among different provenances,Beifang Yuanyi(Northern Horticulture),(5):20-25(王佩兰,许德禄,张斌,李志辉,2014,钩栗种源间幼苗生长状况和光合特性比较研究,北方园艺,(5):20-25)
Wang Y.,Liu X.W.,Liu X.S.,Deng Y.H.,and Jiang Y.,2016Comparison experiment of root cutting at seedling stage in different provenances of Castanopsis tibetana Hance,Beifang Yuanyi(Northern Horticulture),(4):6-11(王勇,刘晓蔚,刘雄盛,邓玉华,蒋燚,2016,钩栗不同种源间苗期切根处理比较试验,北方园艺,(4):6-11)
Wu B.H.,2015,Structural analysis on natural community o Castanopsis tibetana in Youxi Qipanding,Lvse Keji(Journa of Green Science and Technology),(7):55-58(吴碧华,2015尤溪棋盘顶钩栲天然林群落结构分析,绿色科技,(7)55-58)
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