苍耳子标准汤剂质量研究
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摘要
为了更好地评价不同产地苍耳子标准汤剂的出膏率、绿原酸含量及含量转移率,对苍耳子标准汤剂的质量进行了研究。采用日本Shimadzu LC-20A型高效液相色谱仪,Agilent Zorbax Eclipse C_(18)色谱柱(2.1 mm×250 mm,5.0μm),以乙腈-磷酸为流动相,柱温为28℃,流速为1 mL/min,检测波长为327 nm,建立了苍耳子标准汤剂中绿原酸含量的测定方法。结果表明:15批苍耳子标准汤剂出膏率的均值为4.30%,均值±3SD为3.28%～5.32%;绿原酸含量均值为3.44 mg/g,均值±3SD为0～7.16 mg/g;绿原酸含量转移率均值为36.54%,均值±3SD为0.66～72.42%;不同产地苍耳子的出膏率较为稳定,但绿原酸含量及含量转移率差异较大。经方法学考察,苍耳子标准汤剂中绿原酸含量测定方法稳定,具有较高的重复性、稳定性及可信度,可用于苍耳子标准汤剂的质量评价,为苍耳子配方颗粒后续研究及大规模生产提供数据支持。
In order to better evaluate the extraction rate, chlorogenic acid content and content transfer rate of Xanthii Fructus standard decoction in different producing areas, the quality of standard decoction of Xanthii Fructus is studied. The Japanese Shimadzu LC-20 A high performance liquid chromatograph and Agilent Zorbax Eclipse C_(18) column(2.1 mm × 250 mm, 5.0 μm) are applied in the method. The mobile phase is acetonitrile-phosphoric acid solution, the column temperature is 28 ℃, the flow rate is 1 mL/min, and the detection wavelength is 327 nm. A method is established for determining the content of chlorogenic acid in Xanthii Fructus standard decoction. The results show that the average yield of 15 batches of Xanthii Fructus standard decoction is 4.30%, and the mean value ±3 SD ranges from 3.28% to 5.32%.The average chlorogenic acid content is 3.44 mg/g, and the mean ±3 SD ranges from 0 to 7.16 mg/g. The average chlorogenic acid content transfer rate is 36.54%, and the mean value ±3 SD ranges from 0.66 to 72.42%. The results show that the extraction rate of Xanthii Fructus in different habitats is stable, and the chlorogenic acid content and content transfer rate are different. In view of methodology, the method for the determination of chlorogenic acid in Xanthii Fructus standard decoction is stable with high repeatability, stability and credibility, and can be used for quality evaluation of Xanthii Fructus standard decoction. This method provides data support for the follow-up research and mass production of Xanthii Fructus formula granules.
In order to better evaluate the extraction rate, chlorogenic acid content and content transfer rate of Xanthii Fructus standard decoction in different producing areas, the quality of standard decoction of Xanthii Fructus is studied. The Japanese Shimadzu LC-20 A high performance liquid chromatograph and Agilent Zorbax Eclipse C_(18) column(2.1 mm × 250 mm, 5.0 μm) are applied in the method. The mobile phase is acetonitrile-phosphoric acid solution, the column temperature is 28 ℃, the flow rate is 1 mL/min, and the detection wavelength is 327 nm. A method is established for determining the content of chlorogenic acid in Xanthii Fructus standard decoction. The results show that the average yield of 15 batches of Xanthii Fructus standard decoction is 4.30%, and the mean value ±3 SD ranges from 3.28% to 5.32%.The average chlorogenic acid content is 3.44 mg/g, and the mean ±3 SD ranges from 0 to 7.16 mg/g. The average chlorogenic acid content transfer rate is 36.54%, and the mean value ±3 SD ranges from 0.66 to 72.42%. The results show that the extraction rate of Xanthii Fructus in different habitats is stable, and the chlorogenic acid content and content transfer rate are different. In view of methodology, the method for the determination of chlorogenic acid in Xanthii Fructus standard decoction is stable with high repeatability, stability and credibility, and can be used for quality evaluation of Xanthii Fructus standard decoction. This method provides data support for the follow-up research and mass production of Xanthii Fructus formula granules.
引文
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[11]朵睿,刘玉红,王明奎,等.HPLC同时测定苍耳子中6个化学成分的含量[J].药物分析杂志,2013,33(1):78-82.DUO Rui,LIU Yuhong,WANG Mingkui,et al.HPLC simultaneous determination of the content of 6constituents in Fructus Xanthii[J].Chinese Journal of Pharmaceutical Analysis,2013,33(1):78-82.
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[14]戴岳,毕培曦,陈耀邦.苍耳子对速发型过敏反应的抑制作用[J].中国野生植物资源,2002,26(6):61-64.DAI Yue,BU Peixi,CHEN Yaobang.Inhibitory effect of Xanthium sibiricum Fruits on immediate allergic reactions[J].Chinese Wild Plant Resources,2002,26(6):61-64.
[15]赵传胜.苍耳子及其炮制品抗菌作用实验研究[J].时珍国医国药,2002,13(9):522.ZHAO Chuansheng.Study on Xanthium sibiricum and its baked one antibiotic functions[J].Lishizhen Medicine and Materia Medica Research,2002,13(9):522.
[16]潘菊花,王玉琳,谢明仁,等.苍耳子提取物对S180荷瘤小鼠肿瘤生长的抑制剂免疫功能的影响[J].中国临床研究,2013,26(4):317-319.PAN Juhua,WANG Yulin,XIE Mingren,et al.Inhibitory effect of xanthium extract on S180cells growth and the impact on immune functions in tumor-bearing mice[J].Chinese Journal of Clinical Research,2013,26(4):317-319.
[17]SEUNG H,WANG Z Q,WANG H,et al.Xanthium strumarium as an inhibitor ofα-glucosidase protein tyrosine phospatase 1βprotein glycation and ABTS+for diabetic and its complication[J].Molecules,2016,21:1241-1253.
[18]熊颖.苍耳子免疫抑制活性部位初步研究[D].广州:广州中医药大学,2006.XIONG Ying.The Study on Immunosuppression Active Site of Fructus Xanthii[D].Guangzhou:Guangzhou University of Traditional Chinese Medicine,2006.
[2]郭亚红,李家买,潘炯光,等.苍耳子中挥发油的研究[J].中国中药杂志,1994,19(4):235-236.
[3]姜海,张妍妍,张颖,等.苍耳子化学成分研究[J].中医药信息,2016,33(3):8-10.JIANG Hai,ZHANG Yanyan,ZHANG Ying,et al.Isolation and identification of chemical constituents from the fruit of Xanthium sibiricum Patr.[J].Information on Traditional Chinese Medicine,2016,33(3):8-10.
[4]PIACENTE S,PIZZA C,TOMMASI N D,et al.Sesquiterpene and diterpene glycosides from Xanthium spinosum[J].Phytochemistry,1996,41(41):1357-1360.
[5]JIANG H,YANG L,LIU C,et al.Four new glycosides from the fruit of Xanthium sibiricum Patr.[J].Molecules,2013,18(18):12464-12473.
[6]QIN L,HAN T,LI H,et al.A new thiazinedion from Xanthium strumarium[J].Fitoterapia,2006,77(3):245-246.
[7]姜海,杨柳,邢绪东,等.苍耳子的化学成分研究[J].中草药,2017,48(1):47-51.JIANG Hai,YANG Liu,XING Xudong,et al.Chemical constituents from fruits of Xanthium sibiricum[J].Chinese Traditional and Herbal Drugs,2017,48(1):47-51.
[8]韩燕全,洪燕,夏伦祝,等.UPLC指纹图谱技术结合毒性成分含量优选苍耳子的炮制工艺[J].中国中药杂志,2014,39(7):1248-1254.HAN Yanquan,HONG Yan,XIA Lunzhu,et al.Optimization of processing technology for Xanthii Fructus by UPLC fingerprint technique and contents of toxicity ingredient[J].China Journal of Chinese Materia Medica,2014,39(7):1248-1254.
[9]汪洋.中药苍耳子的毒性物质基础及中毒机制研究[D].上海:第二军医大学,2010.WANG Yang.Investigation of the Toxic Constituents and Toxicological Mechanism of Xanthii Fructus[D].Shanghai:The Second Military Medical University,2010.
[10]朵睿,陈燕,刘玉红,等.HPLC测定苍耳子中羧基苍术苷和苍术苷含量[J].中国中药杂志,2012,37(15):2313-2316.DUO Rui,CHEN Yan,LIU Yuhong,et al.Determination of carboxyatrac-tyloside and atractyloside in Xanthii Fructus by HPLC[J].China Journal of Chinese Materia Medica,2012,37(15):2313-2316.
[11]朵睿,刘玉红,王明奎,等.HPLC同时测定苍耳子中6个化学成分的含量[J].药物分析杂志,2013,33(1):78-82.DUO Rui,LIU Yuhong,WANG Mingkui,et al.HPLC simultaneous determination of the content of 6constituents in Fructus Xanthii[J].Chinese Journal of Pharmaceutical Analysis,2013,33(1):78-82.
[12]邱玉玲,代英辉,王东,等.HPLC法测定苍耳子中苍耳子噻嗪双酮苷的含量[J].沈阳药科大学学报.2010,4(4):306-310.QIU Yuling,DAI Yinghui,WANG Dong.et al.Determination of content of xanthiside from fruit of Xanthiunm sibiricum by HPLC[J].Journal of Shenyang Pharmaceutical University,2010,4(4):306-310.
[13]HAN T,LI H L,ZHANG Q Y,et al.Bioactivity-guided fractionation for anti-inflammatory and analgesic properties and constituents of Xanthium strumarium L.[J].Phytomedicine,2007,14(12):825-829.
[14]戴岳,毕培曦,陈耀邦.苍耳子对速发型过敏反应的抑制作用[J].中国野生植物资源,2002,26(6):61-64.DAI Yue,BU Peixi,CHEN Yaobang.Inhibitory effect of Xanthium sibiricum Fruits on immediate allergic reactions[J].Chinese Wild Plant Resources,2002,26(6):61-64.
[15]赵传胜.苍耳子及其炮制品抗菌作用实验研究[J].时珍国医国药,2002,13(9):522.ZHAO Chuansheng.Study on Xanthium sibiricum and its baked one antibiotic functions[J].Lishizhen Medicine and Materia Medica Research,2002,13(9):522.
[16]潘菊花,王玉琳,谢明仁,等.苍耳子提取物对S180荷瘤小鼠肿瘤生长的抑制剂免疫功能的影响[J].中国临床研究,2013,26(4):317-319.PAN Juhua,WANG Yulin,XIE Mingren,et al.Inhibitory effect of xanthium extract on S180cells growth and the impact on immune functions in tumor-bearing mice[J].Chinese Journal of Clinical Research,2013,26(4):317-319.
[17]SEUNG H,WANG Z Q,WANG H,et al.Xanthium strumarium as an inhibitor ofα-glucosidase protein tyrosine phospatase 1βprotein glycation and ABTS+for diabetic and its complication[J].Molecules,2016,21:1241-1253.
[18]熊颖.苍耳子免疫抑制活性部位初步研究[D].广州:广州中医药大学,2006.XIONG Ying.The Study on Immunosuppression Active Site of Fructus Xanthii[D].Guangzhou:Guangzhou University of Traditional Chinese Medicine,2006.