UHPLC-QTOF-MSMS比较两种不同基原火炭母的化学成分
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摘要
为评价不同基原火炭母药材的质量差异,本文采用超高效液相色谱-四极杆飞行时间质谱联用(UHPLC-QTOF-MSMS)技术研究火炭母原变种和硬毛火炭母(变种)的化学成分。结果表明:酚酸类、黄酮类成分是火炭母原变种及硬毛火炭母的主要成分;通过与标准品对照,没食子酸、绿原酸、鞣花酸和槲皮苷等4个成分被准确鉴定;其余21个峰通过一级质谱精确质荷比和二级质谱碎片、结合数据库匹配及参考文献等进行结构鉴定。这一分析方法快捷方便,可快速区别火炭母原变种和硬毛火炭母中的化学成分,为全面研究火炭母药材的质量标准和品质评价等提供科学依据。
In order to evaluate the quality in Polygonum chinense var.chinense and Polygonum chinense var.hispidum from different varieties,the ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-QTOF-MSMS) method was used to evaluate the difference of chemical constituents on Polygonum chinense.The results showed that polyphenols and flavonoids were the main components of both extracts,and four compounds were identified as gallic acid,chlorogenic,ellagic acid and quercitrin based on the reference standards,and 21 compounds were identified or tentatively characterized in P.chinense according to the accurate m/z value analysis of TOF-MS data,and QTOF-MSMS fragment analysis and spectral data from references.This method with the characteristics of fast,sensitivity and efficient,could provide reference for further study in P.chinense,as well as provide scientific basis for the quality standarand research and quality assessment.
In order to evaluate the quality in Polygonum chinense var.chinense and Polygonum chinense var.hispidum from different varieties,the ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-QTOF-MSMS) method was used to evaluate the difference of chemical constituents on Polygonum chinense.The results showed that polyphenols and flavonoids were the main components of both extracts,and four compounds were identified as gallic acid,chlorogenic,ellagic acid and quercitrin based on the reference standards,and 21 compounds were identified or tentatively characterized in P.chinense according to the accurate m/z value analysis of TOF-MS data,and QTOF-MSMS fragment analysis and spectral data from references.This method with the characteristics of fast,sensitivity and efficient,could provide reference for further study in P.chinense,as well as provide scientific basis for the quality standarand research and quality assessment.
引文
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2 Ren HC,Wan DR,Gu J,et al.Chemical constituents from Polygonum chinensis L.[J].Nat Prod Res Dev(天然产物研究与开发),2012,24:1387-1389+1411.
3 Cai JJ,Zeng CY,Mei QX.Research progress on chemical constituents and pharmacological effects of Polygonum chinensis L.[J].Asia-Pac Trad Med(亚太传统医药),2014,10(24):32.
4 Guangdong Food And Drug Administration.Guangdong traditional Chinese medicine standards:Vol 1(广东省中药药材标准:第1册)[M].Guangzhou:Guangdong Science and Technology Press,2004.
5 Department of Health,the government of the Hongkong special administrative region,The People’s Republic of China.Hongkong Chinese materia medica standards:Vol 7(香港中药材标准:第7册)[S].Hongkong,2017.
6 He Y,Yu SS,Dai YF,et al.Study on ginsenosides in white ginseng and Dali ginseng by RRLC-Q-TOFMS/MS[J].J Chin Mass Spectr Soc(质谱学报),2014,35:311-316.
7 Yang LM,Yang LJ,Jia P,et al.HPLC-Q-TOF-MS/MS-based analysis of chemical constituents in ChoerospOndiatis fructus[J].Acad J Second Mil Med Univ(第二军医大学学报),2016,37:159-166.
8 Pedro M,Luca C,Chiara D,et al.Rapid and comprehensive evaluation of (Poly) phenolic compounds in pomegranate(Punica granatum L.) juice by UHPLC-MsN[J].Molecules,2012,17:14821-14840.
9 Yan Y,Zhao H,Zou LS,et al.Difference of chemical constituents in Eucommiae cortex from different habitats by LC-QTOF MS/MS[J].Chin J Chin Mater Med(中国中药杂志),2017,42:2730-2737.
10 Wang YQ,Si JY,Liu XM,et al.Isolation,identification and quantitative determination of elagic acid and brevifolin carboxylic acid in duchesnea indica by RP-HPLC[J].Nat Prod Res Dev(天然产物研究与开发),2008,20:667-671.
11 Zhou BH,Yi HL,Guo XX,et al.Preliminary analysis of tannin-related constituents in Pomegranate peel by HPLC-ESI-MS[J].China Pharm(中国药师),2015,18:201-204.
12 Eldahshan OA,Ayoub NA,Singab,et al.Potential superoxide anion radical scavenging activity of Doum Palm(Hyphaene thebaica L.) leaves extract[J].Rec Nat Prod,2008,2(3):83-93.
13 Wang Q,Lu ZW,Liu YH,et al.Rapid analysis on phenolic compounds in Rheum palmatum based on UPLC-Q-TOF/MSE combined with diagnostic ions filter[J].Chin J Chin Mater Med(中国中药杂志),2017,42:1922-1931.
14 Liu DY,Shi XF,Li C,et al.Chemical constituents of flavonoids in pine needles of Cedrus deodara[J].Chin Tradit Herb Drugs(中草药),2011,42:631-633.
15 Zeng ML,Shen NT,Wu SW,et al.Analysis on chemical constituents in Tetrastigma hemsleyanum by UPLC-Triple-TOF/MS[J].Chin Tradit Herb Drugs(中草药),2017,48:874-883.
16 Jin LZ,Qing MC,Shou ZL,et al.Study on metabolism of scutellarin in rats by HPLC-MS and HPLC-NMR[J].J Asian Nat Prod Res,2003,5:249-256.
17 Bai NS,He K,Marc R,et al.Active compounds from Lagerstroemia speciosa,insulin-like glucose uptake-stimulatory/inhibitory and adipocyte differentiation-inhibitory actitvties in 3T3-L1 cells[J].J Agric Food Chem,2008,56:11668-11674.
18 Yi F,Hu LH.Two DNA topoisomerase inhibitors in Camptotheca acuminata[J].Chin J Nat Med(中国天然药物),2005,1:25-31.
19 Yu JY,Wang YH,Liu GQ,et al.Identification of flavonoids from Thlaspi arvense L.by HPLC-ESI-MS/MS[J].Chin Tradit Pat Med(中成药),2015,37:556-561.
20 Yang L.Research development of pharmacology activities of quercitrin[J].Asia-Pac Trad Med(亚太传统医药),2015,11(6):61-65.