噬菌体在检测食源性病原菌中的应用研究进展
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摘要
食源性病原菌是导致食源性疾病的主要原因之一,现代食品工业的迅猛发展对食品中病原菌的快速检测提出了更高的要求。噬菌体作为地球上种类最丰富的微生物之一,能够侵染细菌。研究表明,噬菌体不仅具备结构简单、特异性强、价格低廉等特性,而且具有能够区分活细菌和死细菌的能力,以及容易与其他传统检测方法相结合等优势,噬菌体及其产物为食源性病原菌的检测提供了新的思路。近年来,噬菌体与免疫学、分子生物学和纳米科学等学科结合形成的新型快速检测方法已成为国际研究热点。本文就噬菌体检测食源性病原菌的原理及应用进行分类综述和分析。
Foodborne pathogenic bacteria are recognized as one of the major causes of food-borne diseases. With the fast development of modern food industry, higher requirements have been placed on the rapid detection of pathogens in foods. Considered as the most abundant organism on Earth, bacteriophage can infect bacterial hosts naturally. Current research has documented that phage not only has the characteristics of simple structure, high specificity and low price, but also has the ability to distinguish between living and dead bacteria and is easy to combine with traditional methods for detecting bacterial pathogens in foods. Phages and phage-encoded products can provide a new tool for the detection of foodborne pathogens. More recently, many rapid phage-based detection methods have been developed, especially the application of phage in conjunction with immunology, molecular biology and nanoscale science. In this paper, the principles and applications of phage-based methods for the detection of foodborne pathogens are reviewed and analyzed.
Foodborne pathogenic bacteria are recognized as one of the major causes of food-borne diseases. With the fast development of modern food industry, higher requirements have been placed on the rapid detection of pathogens in foods. Considered as the most abundant organism on Earth, bacteriophage can infect bacterial hosts naturally. Current research has documented that phage not only has the characteristics of simple structure, high specificity and low price, but also has the ability to distinguish between living and dead bacteria and is easy to combine with traditional methods for detecting bacterial pathogens in foods. Phages and phage-encoded products can provide a new tool for the detection of foodborne pathogens. More recently, many rapid phage-based detection methods have been developed, especially the application of phage in conjunction with immunology, molecular biology and nanoscale science. In this paper, the principles and applications of phage-based methods for the detection of foodborne pathogens are reviewed and analyzed.
引文
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[31]CAPPILLINO M,SHIVERS R P,BROWNELL D R,et al.Sample6 DETECT/L:an in-plant,in-shift,enrichment-free Listeriaenvironmental assay[J].Journal of AOAC International,2015,98(2):1-16.DOI:10.5740/jaoacint.14-213.
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